4,233 results

Showing: 1821 - 1840 of 4233 results

Results per page:

Sort results by:

pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Rab5CA(Q79L)

Plasmid

#35138

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe puro c-met WT

Plasmid

#17493

Depositor

Joan Brugge

Article

Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

Insert

c-Met (Met Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-MT GLI2 FL

Plasmid

#17648

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-CRY2clust

Plasmid

#105624

Purpose

Expresses improved CRY2PHR homo-oligomerization

Depositor

Won Do Heo

Article

Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10

Insert

mCherry-CRY2PHR-9aa (CRY2 Mustard Weed, Human)

Tags

9 amino acids

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23b-15F11-HA-mEGFP-6xHis

Plasmid

#129593

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCS2-hDKK1-flag

Plasmid

#15494

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK1 (DKK1 Human)

Tags

flag

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-CRBN-pGK-HYG

Plasmid

#107380

Purpose

Mammalian expression of FLAG-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107

Plasmid

#67639

Purpose

Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52 and pTDH3 (aka GAP promoter)

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-Sos1

Plasmid

#32920

Depositor

Dafna Bar-Sagi

Article

Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

Insert

SOS1 (SOS1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-sfGFP

Plasmid

#85482

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP

Tags

6x His

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PGK GFP Puro (w509-5)

Plasmid

#19070

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pR26 CAG AsiSI/MluI

Plasmid

#74286

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pdCas9

Plasmid

#46569

Purpose

Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9
CRISPR array

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge