4,174 results

1. pWPXL-Sox9

   Plasmid

   #36979

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox9 (Sox9 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PP7-2xmCherry-CAAX

   Plasmid

   #74925

   Purpose

   Expresses PP7-2xmCherry-CAAX

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   PP7-2xmCherry-CAAX
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS598 EF1alpha-EGFPcre

   Plasmid

   #11923

   Purpose

   Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVax1-hVEGF165

   Plasmid

   #74466

   Purpose

   expression of hVEGF165 in mammalian cells

   Depositor

   Loree Heller

   Article

   Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

   Insert

   vascular endothelial growth factor 165 (VEGFA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6.3/V5-DEST-GFP

   Plasmid

   #40125

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 iCasper T2A HO1

   Plasmid

   #64278

   Purpose

   Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PU6::unc-119_sgRNA

   Plasmid

   #46169

   Purpose

   unc-119 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   unc-119 targeting sgRNA (unc-119 Synthetic)
   

   Use

   CRISPR

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

    Plasmid

    #65417

    Purpose

    AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

    Depositor

    Bryan Roth

    Article

    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

    Has Service

    AAV8

    Insert

    hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)
    

    Use

    AAV

    Tags

    HA and mCitrine

    Promoter

    hSyn

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pShuttle

    Plasmid

    #16402

    Purpose

    Shuttle vector for use in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1-Nanog

    Plasmid

    #28221

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Nanog (NANOG Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG148 FRB-NFAST

    Plasmid

    #130812

    Purpose

    Expresses FRB-NFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FRB-NFAST
    

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ADAM10-HA

    Plasmid

    #65106

    Purpose

    Expression of HA tagged ADAM10 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    ADAM10 (ADAM10 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMo130-TelR

    Plasmid

    #50799

    Purpose

    Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

    Depositor

    Kim Lee Chua

    Article

    Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

    Insert

    tellurite-resistance cassette
    

    Use

    Suicide vector

    Promoter

    PCs12

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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