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pCMV5-ALK2 wt

Plasmid

#11741

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pGEMABC

Plasmid

#45398

Depositor

Katsu Murakami

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Inserts

rpoA (rpoA Escherichia coli)
rpoB (rpoB Escherichia coli)
rpoC (rpoC Escherichia coli)

Expression

Bacterial

Promoter

None and T7

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-HA-ERK5-FL

Plasmid

#31817

Depositor

Astar Winoto

Article

Kasler et al Mol Cell Biol. 2000 Nov;20(22):8382-9.

Insert

Erk5 (Mapk7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-kcnk18

Plasmid

#209000

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk18 (kcnk18 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBz15_Strep_Rubisco

Plasmid

#140839

Purpose

wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco; CbbL, CbbS

Tags

Strep II

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pVH41-Tier1-PhCMV-GLuc

Plasmid

#169544

Purpose

Tier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Gaussia Luciferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2374-Tier1-PhCMV-Gal4_VP16

Plasmid

#169582

Purpose

Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

GAL4-transactivator

Expression

Mammalian

Promoter

PhCMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA6

Plasmid

#19459

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA6 (HSPA6 Human)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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LMNA_mScarlet-I_Donor

Plasmid

#178092

Purpose

HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA-mScarlet-I HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

Plasmid

#218634

Purpose

Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

delPYD (I125M-END)

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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DGK zeta

Plasmid

#35410

Depositor

Robert Lefkowitz, Stephen Prescott

Article

Nelson et al Science. 2007 Feb 2;315(5812):663-6.

Insert

DGK zeta (DGKZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pTS1017-Tier1-OTetO7-PCMVmin-SEAP

Plasmid

#169586

Purpose

Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5_Ptet

Plasmid

#179336

Purpose

gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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p663-UBC-miniTurbo-V5-MAPK15_IDG-K

Plasmid

#170581

Purpose

Gateway destination clone of MAPK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-MAPK15 (MAPK15 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_Lyn11-mTurquoise2-iLID

Plasmid

#161000

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Lyn11-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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NHA-GW182

Plasmid

#36438

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 254-1962 only

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only
GST-NDP52

Plasmid

#171422

Purpose

For the purifcation of NDP52 protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

NDP52 (CALCOCO2 Human)

Expression

Bacterial

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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JH03

Plasmid

#73512

Purpose

mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mKate2::PH::tbb-2

Tags

mKate2

Promoter

mex-5

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

Plasmid

#214989

Purpose

Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

CRISPR

Tags

3xFLAG

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pVH231-Tier1-PmPGK1-mTagBFP2

Plasmid

#169520

Purpose

Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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