12,610 results

1. pCS2+ YFP-Smo

   Plasmid

   #41086

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   smoothened homolog (Smo Mouse)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   YFP inserted after Smo signal sequence

   Promoter

   Available since

   Nov. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-24p3/LCN2-Luc

   Plasmid

   #25463

   Depositor

   Sarah Gaffen

   Article

   Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

   Insert

   24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   June 25, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-Akt-DD

   Plasmid

   #39536

   Depositor

   Julian Downward

   Article

   Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

   Insert

   Akt (Akt1 Mouse)
   

   Use

   Tags

   6G linker, EGFP, and HA

   Expression

   Mammalian

   Mutation

   DD = T308D and S473D

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ad:2CMV_A2R2-N25K3

   Plasmid

   #122242

   Purpose

   Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

   Depositor

   Corey Neu

   Article

   Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

   Inserts

   C-terminal end of Nesprin-3 (Syne3 Mouse)
   Actinin alpha 2 (Actn2 Mouse)
   

   Use

   Adenoviral

   Tags

   Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ad:2CMV_A2R2-N25Ctr

   Plasmid

   #122243

   Purpose

   Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

   Depositor

   Corey Neu

   Article

   Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

   Inserts

   C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
   Actinin alpha 2 (Actn2 Mouse)
   

   Use

   Adenoviral

   Tags

   Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

   Expression

   Mammalian

   Mutation

   deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…

   Promoter

   CMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-ZZ-TEV-SNAPf-BICDR1

   Plasmid

   #111858

   Purpose

   N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICDR1 (Bicdl1 Mouse)
   

   Use

   Tags

   His-tag, SNAPf-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   R250Q, Optimised for Sf9 expression

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-c-Myc-IP

   Plasmid

   #15921

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiX2-PURO-shLKB1-Ms

   Plasmid

   #61231

   Purpose

   Lentivirus expressing shRNA to mouse LKB1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   shLkb1 (Ms/Hu) (Stk11 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

   Plasmid

   #159911

   Purpose

   Mutagenesis of Kcnq3

   Depositor

   Larry Zweifel

   Insert

   Kcnq3 (Kcnq3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nanog5P reporter

    Plasmid

    #16337

    Depositor

    Austin Cooney

    Article

    Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

    Insert

    Nanog promoter (Nanog Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Nanog promoter

    Available since

    Dec. 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. REEP5-mEmerald

    Plasmid

    #186964

    Purpose

    Express REEP5/DP1 with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Reep5 (Reep5 Mouse)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GEARBOCS-SPARCL1-N-mCherryTag

    Plasmid

    #218186

    Purpose

    To tag Hevin with mCherry at the N-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-puro-N3FLAG-MYB-FL

    Plasmid

    #105596

    Purpose

    retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Full length MYB (mouse) (Myb Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-AR

    Plasmid

    #111215

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    AR (Ar Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTY-EF1a-Hoxa9-p2a-MeisI-p2a-GFP

    Plasmid

    #61738

    Purpose

    Express Hoxa9 and MeisI to induce leukemic transformation. GFP label transduced cells.

    Depositor

    Yi Zhang

    Article

    He et al Blood. 2011 Apr 7;117(14):3869-80. doi:

    Insert

    Hoxa9-MeisI-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-FLEX-EGFP-mir30(Scn9a)

    Plasmid

    #79672

    Purpose

    Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP (Scn9a Mouse, jellyfish)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZZMP09

    Plasmid

    #231754

    Purpose

    Mammalian expression of SPO11

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    SPO11 (Spo11 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZZMP26

    Plasmid

    #231755

    Purpose

    Mammalian expression of TOP6BL

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    TOP6BL (Gm960 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid

    #216695

    Purpose

    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJFRC5-5XUAS-IVS-mCD8::GFP

    Plasmid

    #26218

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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