We narrowed to 11,153 results for: streptococcus -(crispr) -(cas9)
-
Plasmid#134913PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyPromoterU6Available SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only
-
M_ST1n_VPR
Plasmid#63799PurposeST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vectorDepositorInsertST1-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-dgRNA-CAG-MPH
Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorAvailable SinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRExpressionMammalianPromoterPgkAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAG-CRErec
Plasmid#119971PurposeExpresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE proteinDepositorInsertGAG-nlsCRErec
TagsnlsExpressionMammalianPromoterhCMVAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVSV-G
Plasmid#138479PurposeExpresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.DepositorInsertVSV-G envelop protein
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-S12
Plasmid#84031PurposeTo episomally express codon optimized Cas9 and chimeric guide RNADepositorInsertshSpCas9
eGFP
Tags3x FLAGExpressionMammalianPromoterCMV and CMV (downstream of F2A self-cleaving pept…Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cas9
Plasmid#198275PurposeFor loading SpCas9 into PVCs; also contains PVC regulatory genesDepositorInsertPdp1_NTD-Cas9
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRExpressionMammalianPromoterhU6Available SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64
Plasmid#180263PurposeLentiviral expression vector for dCas9-VP64 in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
Tags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag::UbvG08 I44A, deltaGG
Plasmid#74939PurposeUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1DepositorInsertUbiquitin
TagsFlagExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable SinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZR071_SFFV-dCas9-mCherry-KRAB
Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Cas9-TagBFP
Plasmid#236725PurposeCloning vector Cas9-TagBFPDepositorInsertCas9-TagBFP
UseCRISPRAvailable SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-B2M-miniGag-Cas9
Plasmid#228958PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRExpressionMammalianAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-cas9-2A-mCherry
Plasmid#107592PurposeAn entry vector with cas9 -2A-mCherryDepositorInsertcas9-2A-mCherry
UseCRISPRTags2A-mCherryAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralMutationD64V mutation within the integrase within PolAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only