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Showing: 3721 - 3760 of 84526 results

  12. attB53_SNCB-_attB53

    Plasmid
    #183615
    Purpose
    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183615-plasmid-map-sequence-id-366916

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  15. pCEP4_HLA-cmyc-GRM3 C127A T174A-VC

    Plasmid
    #140457
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140457-plasmid-map-sequence-id-292299

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  17. pCMV_NFL(K468TAG)-FLAG

    Plasmid
    #182658
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K468TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182658-plasmid-map-sequence-id-363090

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  18. pCMV_NFL(K211TAG)-FLAG

    Plasmid
    #182655
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K211TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182655-plasmid-map-sequence-id-363059

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  19. pCMV_NFL(R438TAG)-FLAG

    Plasmid
    #182657
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    R438TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182657-plasmid-map-sequence-id-363055

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  20. pCMV_FLAG-NFL(K468TAG)

    Plasmid
    #182663
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K468 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K468TAG
    Promoter
    CMV
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182663-plasmid-map-sequence-id-362921

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  22. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid
    #136884
    Purpose
    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)
    Depositor
    Moritz Mall
    Article
    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi
    Insert
    engrailed repressor domain (en Fly)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    136884-plasmid-map-sequence-id-272359

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  25. ARB367

    Plasmid
    #124050
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124050-plasmid-map-sequence-id-252515

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  28. pMCh-1498

    Plasmid
    #82427
    Purpose
    Plasmid for expression of NHA-tagged Nematostella vectensis GW182 11W11A in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 11W11A
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    Trp 1167, 1183, 1227, 1309, 1391, 1453, 1574, 158…
    Promoter
    CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82427-plasmid-map-sequence-id-156194

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  29. pMCh-1539

    Plasmid
    #82432
    Purpose
    Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 chimera
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    EF1A
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82432-plasmid-map-sequence-id-156170

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  30. pMCh-1537

    Plasmid
    #82430
    Purpose
    Plasmid for expression of NHA-tagged human-Nematostella GW182 chimera in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    hs-nvGW182 chimera
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …
    Promoter
    CMV
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82430-plasmid-map-sequence-id-156190

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  32. pcdna3 T7 Jip1b(471-707)(C478S and C699T)

    Plasmid
    #52132
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and changed C478S and C6…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. mutpRL-TK 1x perfect to bulged siRNA

    Plasmid
    #40765
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to bulged target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. mutpRL-TK 1x perfect to seed siRNA

    Plasmid
    #40766
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid
    #40767
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed plus 13-16 target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 3721 - 3760 of 84526 results