Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,610 results for: SUP

Showing: 4651 - 4700 of 5610 results

  1. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

    Enlarge

  2. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

    Enlarge

  3. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

    Enlarge

  4. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI site removed
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

    Enlarge

  5. pLenti CMV GSE T329A Hygro (w189-4)

    Plasmid
    #22255
    Depositor
    Eric Campeau
    Insert
    Genetic suppressor element #22, T329A
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Threonine 329 is mutated to Alanine (p53 nomencat…
    Promoter
    Available since
    Dec. 15, 2009
    Availability
    Academic Institutions and Nonprofits only
    22255-plasmid-map-sequence-id-10195

    Enlarge

  6. C67m

    Plasmid
    #120981
    Purpose
    MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bxb1 integrase - mScarlet-i - LAA tag
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120981-plasmid-map-sequence-id-236036

    Enlarge

  7. Paxillin_PSCFP2_ mEOS2

    Plasmid
    #114192
    Purpose
    studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling
    Depositor
    Aleksandra Radenovic
    Insert
    Paxillin (PXN Chicken)
    Use
    Tags
    PS-CFP2 and mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114192-plasmid-map-sequence-id-225613

    Enlarge

  8. PB CAG-R-3xSV40pA-R-td-sfGFP

    Plasmid
    #177150
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre / rox ; piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177150-plasmid-map-sequence-id-348857

    Enlarge

  10. PB CAG-R-bpA-3xSV40pA-R-td-sfGFP

    Plasmid
    #133579
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133579-plasmid-map-sequence-id-274429

    Enlarge

  11. UC16m

    Plasmid
    #121038
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gQi Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121038-plasmid-map-sequence-id-236383

    Enlarge

  12. UC17m

    Plasmid
    #121039
    Purpose
    MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gV1 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121039-plasmid-map-sequence-id-236352

    Enlarge

  13. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

    Enlarge

  14. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

    Enlarge

  16. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50468-plasmid-map-sequence-id-75484

    Enlarge

  17. SUV39H2

    Plasmid
    #25115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H2_04 (SUV39H2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. RAB14

    Plasmid
    #25519
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB14 (RAB14 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165574-plasmid-map-sequence-id-340976

    Enlarge

  21. SIRT5

    Plasmid
    #25487
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIRT5 (SIRT5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25487-plasmid-map-sequence-id-203224

    Enlarge

  22. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

    Plasmid
    #121546
    Purpose
    Super-bright fluorescence protein for tracking and visualization
    Depositor
    Matthias Prigge
    Insert
    cre-dependent mNeon
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121546-plasmid-map-sequence-id-308125

    Enlarge

  23. pRKVSV-PTEN

    Plasmid
    #50538
    Depositor
    Kenneth Yamada
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    2X VSV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

    Enlarge

  25. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

    Enlarge

  26. pRKVSV-PTEN C124A

    Plasmid
    #50539
    Depositor
    Kenneth Yamada
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    2X VSV
    Expression
    Mammalian
    Mutation
    C124A
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pRKVSV-PTEN G129E

    Plasmid
    #50542
    Depositor
    Kenneth Yamada
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    2X VSV
    Expression
    Mammalian
    Mutation
    G129E
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid
    #50478
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50478-plasmid-map-sequence-id-166302

    Enlarge

  29. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid
    #50479
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50479-plasmid-map-sequence-id-166303

    Enlarge

  32. pGustiv

    Plasmid
    #240335
    Purpose
    Expresses BK polyomavirus genotype IV capsid protein VP1 in yeast. Expression of VP1 (and a GFP reporter) is induced by maltose.
    Depositor
    Christopher Buck
    Inserts
    BKV-IV VP1
    Superfold GFP
    Formaldehyde dehydrogenase 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    FDH1, MAL31, and MAL32
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    240335-plasmid-map-sequence-id-478372

    Enlarge

  33. M3-PAmcherry1

    Plasmid
    #114189
    Purpose
    studying clustering of M3 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    Use
    Tags
    3xHA and PAmCherry
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114189-plasmid-map-sequence-id-224304

    Enlarge

  34. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

    Enlarge

  37. alpha3-nAChR shRNA (Alpha-3.29)

    Plasmid
    #86655
    Purpose
    expression of shRNA targeting CHRNA3
    Depositor
    Jaime Modiano
    Insert
    CHRNA3 (alpha3-nAChR) (CHRNA3 Human)
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86655-plasmid-map-sequence-id-180126

    Enlarge

  38. alpha4-nAChR shRNA (Alpha-4.3)

    Plasmid
    #86652
    Purpose
    expression of shRNA targeting CHRNA4
    Depositor
    Jaime Modiano
    Insert
    CHRNA4 (alpha4-nAChR) (CHRNA4 Human)
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1
    Available since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86652-plasmid-map-sequence-id-180128

    Enlarge

  39. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50475-plasmid-map-sequence-id-171072

    Enlarge

  40. pKAR2-Br512g3.1

    Plasmid
    #171200
    Purpose
    Expresses Br512g3.1 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171200-plasmid-map-sequence-id-339557

    Enlarge

  42. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid
    #50458
    Purpose
    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    rM3D-mCherry
    Use
    AAV
    Tags
    HA and mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50458-plasmid-map-sequence-id-162264

    Enlarge

  45. pKAR2-Br512g3.2

    Plasmid
    #171201
    Purpose
    Expresses Br512g3.2 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171201-plasmid-map-sequence-id-339558

    Enlarge

  46. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. SUV39H1

    Plasmid
    #28113
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV39H1 (SUV39H1 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains chromo domain only (amino acid residues …
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4651 - 4700 of 5610 results