We narrowed to 22,307 results for: CRISPR

1. lenti.Cas9.BFP.sgRNA.parental

   Plasmid

   #196715

   Purpose

   Sortable WT-SpCas9 expression and guide cassette (1-vector system).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAK_DR30_EF1a_CasRx_Hygro

   Plasmid

   #134841

   Purpose

   Lentiviral all in one expression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR and Lentiviral

   Tags

   T2A Hygromycin

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FgH1tUTG_hup53_Ex4

   Plasmid

   #85538

   Purpose

   Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Insert

   hup53 Ex4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCHph

   Plasmid

   #185863

   Purpose

   The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker

   Depositor

   Claudia Vickers

   Insert

   PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FgH1tUTG_hup53_Ex5

   Plasmid

   #85539

   Purpose

   Inducible expression of guide RNA (hup53_Ex5) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Insert

   hup53 Ex5
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-spCas9-pZeo

   Plasmid

   #214121

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJCKan

   Plasmid

   #185862

   Purpose

   The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker

   Depositor

   Claudia Vickers

   Insert

   PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDual_dsCas9_BFP

   Plasmid

   #214679

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSaGuide

   Plasmid

   #64710

   Purpose

   Cloning and expression of Staphylococcus aureus guide RNA

   Depositor

   Kiran Musunuru

   Insert

   Staphylococcus aureus guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSmart-Nm-sgRNA-BbsI

    Plasmid

    #49157

    Purpose

    Plasmid for cloning spacer into sgRNA for NmCas9

    Depositor

    James Thomson

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORFE0005

    Plasmid

    #112073

    Purpose

    AtDeadCAS9 D10A H840A without stop codon module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtDeadCAS9 D10A H840A without stop codon
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLK88 - Edit-directing plasmid base

    Plasmid

    #98814

    Purpose

    Edit-directing plasmid base

    Depositor

    Leonid Kruglyak

    Insert

    CAN1.y gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMK

    Plasmid

    #62818

    Purpose

    Bacterial constitutive S. pyogenes Cas9 expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. FgH1tUTG_Bfl-1.1

    Plasmid

    #85536

    Purpose

    Inducible expression of guide RNA (Bfl-1.1) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Insert

    Bfl1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. FgH1tUTG_Bfl-1.2

    Plasmid

    #85537

    Purpose

    Inducible expression of guide RNA (Bfl-1.2) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Insert

    Bfl1.2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDual_dsCas9_Venus

    Plasmid

    #214678

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRR1

    Plasmid

    #166096

    Purpose

    Gal inducible expression of Cas9 WT

    Depositor

    Rebecca Burgess

    Insert

    WT spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid

    #236043

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC67907

    Plasmid

    #211794

    Purpose

    Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas9, GR-GRF-GIF
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. EC67908

    Plasmid

    #211795

    Purpose

    Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas12a, GR-GRF-GIF
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCFD5

    Plasmid

    #73914

    Purpose

    multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    dU6-3:tRNA:gRNA:tRNA:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. p(U6a-BsaI-gRNA)

    Plasmid

    #65955

    Purpose

    Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid

    #159916

    Purpose

    Mutagenesis of Penk

    Depositor

    Larry Zweifel

    Insert

    Penk (Penk Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

    Plasmid

    #159905

    Purpose

    Mutagenesis of Slc32a1

    Depositor

    Larry Zweifel

    Insert

    Slc32a1 (Slc32a1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEA186

    Plasmid

    #187874

    Purpose

    Empty pVS1-based INTEGRATE vector with mCherry cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pKL2310

    Plasmid

    #187875

    Purpose

    Empty pVS1-based INTEGRATE vector with loxP cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

    Plasmid

    #225752

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAW019-2

    Plasmid

    #85614

    Purpose

    Inducible dCas9 integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PxylA (B. megaterium)

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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