We narrowed to 10,496 results for: nar

1. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_6HT-TBP6.7(delta C-term)

   Plasmid

   #112731

   Purpose

   This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(delta C-term)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   delta C-term

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRY-GalVP16 (B695)

   Plasmid

   #92031

   Purpose

   Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   GalBD-VP16AD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRB117.2

   Plasmid

   #181949

   Purpose

   aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   mNG-NarL; mCherry; tetR (narL, AX04_RS23485 narL - E. coli, Synthetic)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial

   Mutation

   See Depositor Comments Below

   Promoter

   mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRB113.2

    Plasmid

    #181948

    Purpose

    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarL-mNG; mCherry; tetR (AX04_RS23485, narL narL - E. coli, Synthetic)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKD317.8

    Plasmid

    #181951

    Purpose

    IPTG-inducible expression of NarX

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NarX-Ptac; lacI-PlacIq

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ

    Plasmid

    #67965

    Purpose

    Indicuble TraJ expression with arabinose.

    Depositor

    Joshua Leonard

    Insert

    TraJ
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJaM

    Plasmid

    #67966

    Purpose

    Inducible TraJ, TraM expression with arabinose.

    Depositor

    Joshua Leonard

    Insert

    TraM, TraJ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pShuttle

    Plasmid

    #67964

    Purpose

    Mobilizible plasmid with EGFP. OriT from the F plasmid.

    Depositor

    Joshua Leonard

    Insert

    OriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNKR1

    Plasmid

    #67968

    Purpose

    High copy number plasmid encoding part of the tra Operon PY-traR

    Depositor

    Joshua Leonard

    Insert

    tra Operon (First Quarter)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLNKR2

    Plasmid

    #67969

    Purpose

    High copy number plasmid encoding part of the tra Operon traC-artA

    Depositor

    Joshua Leonard

    Insert

    tra Operon (second Quarter)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CrBlue-mcherry - pcDNA3.1

    Plasmid

    #64204

    Purpose

    CrBlue fused to mcherry on the C terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    CrBlue
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pDB1751

    Plasmid

    #174013

    Purpose

    pDUAL plasmid expressing GFP from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    GFP
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1 and 41nmt1 promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-Gprotein Gamma 9

    Plasmid

    #42201

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    G protein Gamma 9
    

    Use

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pDB4924

    Plasmid

    #174012

    Purpose

    pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Pil1-mCherry
    

    Use

    Tags

    Pil1, mCherry

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1 and 41nmt1p

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Blue opsin mcherry ---- pcDNA3.1

    Plasmid

    #62932

    Purpose

    Blue opsin tagged with mcherry on the C terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    blue opsin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CrBlue ---- pcDNA3.1

    Plasmid

    #62917

    Purpose

    CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    CrBlue
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pDB4961

    Plasmid

    #174015

    Purpose

    pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Vps15 (ppk19 Fission Yeast)
    

    Use

    Tags

    13Myc, Vps15

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDB4953

    Plasmid

    #174020

    Purpose

    pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg6
    

    Use

    Tags

    GFP, Atg6

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDB4658

    Plasmid

    #174017

    Purpose

    pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg8
    

    Use

    Tags

    GFP, Atg8

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDB4958

    Plasmid

    #174014

    Purpose

    pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg6
    

    Use

    Tags

    CFP, Atg6

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDB4948

    Plasmid

    #174019

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Vps38
    

    Use

    Tags

    Pil1, mCherry, Vps38

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDB4946

    Plasmid

    #174018

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg14 (atg14 Fission Yeast)
    

    Use

    Tags

    Pil1, mCherry, Atg14

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDB4925

    Plasmid

    #174016

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg38(161-190)
    

    Use

    Tags

    Pil1, mCherry, Atg38(161-190)

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRL_GI_PCB28

    Plasmid

    #98745

    Purpose

    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Inserts

    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    ADH1m and PGK1

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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