We narrowed to 23,989 results for: Sis
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Plasmid#99526PurposeN-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-116) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterCUP1Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR(W349R)-mCherry
Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
TagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHEE901-A3A
Plasmid#229044PurposenCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_A215C
Plasmid#177828PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_A215C
ExpressionMammalianMutationA215CPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
ExpressionMammalianMutationC289TPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80Q
Plasmid#118351PurposePlasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK32-Flag
Plasmid#107432PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
ExpressionMammalianMutationA191GPromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-WT
Plasmid#60030PurposeWT CYLD under control of Tet promoter with N-terminal FLAG tagDepositorAvailable SinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE-BR1
Plasmid#160333PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.DepositorInsertBR1
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-9myc-AID*(N)
Plasmid#99527PurposeN-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterCUP1Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
mRFP-mem
Plasmid#55622PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.DepositorInsertmRFP-Mem
TagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-AtUBC8-S-AtUBA1-S
Plasmid#222714PurposeExpression AtUBA1-S and AtUBC8-S in in bacterial cellsDepositorAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKan-PRFA1-9myc-AID*(N)
Plasmid#99528PurposeN-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterRFA1Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-ERK5
Plasmid#53175PurposeExpresses HA tagged human ERK5 from blasticidin resistance retroviral vectorDepositorAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
PBE-NG
Plasmid#229045PurposeModified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
Myc-CYLD-C601A
Plasmid#60027PurposeMyc-tagged CYLD catalytic inactive (C601A) expressionDepositorInsertCylindromatosis (CYLD Human)
TagsMycExpressionMammalianMutationCysteine 601 changed to AlaninePromoterCMVAvailable SinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)Mutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSNV2 CHER NLS cop1at(335-675)
Plasmid#44972DepositorInsertmCherry-NLS-COP1 (COP1 Mustard Weed)
TagsNLS and mCherryExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMV IEAvailable SinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SH3
Plasmid#160331PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.DepositorInsertSH3
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBEC8
Plasmid#187599PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
ExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
NPY-mApple
Plasmid#83498PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP-alpha(740-977)-GFP
Plasmid#100731PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)DepositorInsertAP2A1 (Ap2a1 Mouse)
TagsFKBP and GFPExpressionMammalianMutationLong isoformPromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUB-nHyPer2 (NLS:HyPer2:NLS)
Plasmid#84730PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to NucleiDepositorInsertHyPer2 targeted to nucleus
TagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-FAK-HA-V744G
Plasmid#35040DepositorInsertfocal adhesion kinase (Ptk2 Mouse)
Tags2x HA and mCherryExpressionMammalianMutationGFP-FAK-V744G was generated from GFP-FAK using th…Available SinceMarch 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-PDS3_IMS
Plasmid#112875Purposebinary vector for IMS (Induced Mis-Splicing) of PDS3 in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3
UseCRISPRExpressionPlantAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Htr5b(3.7)-EGFP-sv40
Plasmid#184411PurposeAAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.DepositorInsertMouse Htr5b gene promoter-EGFP (Htr5b Mouse)
UseAAVTagsEGFPExpressionMammalianPromoterMouse Htr5b geneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB6
Plasmid#177657PurposeRecombinant protein synthesis of human DNAJB6DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVneo
Plasmid#134982PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB-nSypHer (NLS:sypHer:NLS)
Plasmid#84731PurposeExpresses sypHer in plant cells-biosensor for pH targeted to NucleiDepositorInsertnSypHer
TagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
TagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB4
Plasmid#177656PurposeRecombinant protein synthesis of human DNAJB4DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-T38M-750-luxAB-DAS+4
Plasmid#49520PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomallyDepositorInsertT38M-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRAS G12V-IRES-ASK1
Plasmid#221074PurposeExpresses NRAS G12V and ASK1 in mammalian cellsDepositorAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SEP-Nanoluc-cyOFP1
Plasmid#141082Purposeprototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEP-Nanoluc-cyOFP1
UseLentiviralMutationDeletion of amino acids DISGG from positions 673 …PromoterEF1αAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:AtFLS2
Plasmid#202186PurposeExpress Arabidopsis thaliana FLS2 gene under UBQ10 promoterDepositorAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only