We narrowed to 9,416 results for: Coli

1. pSmBit-CHIP-K30A

   Plasmid

   #236066

   Purpose

   SmBit-CHIP expression with K30A substitution

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   SmBit-CHIP-K30A (STUB1 Human)
   

   Use

   Luciferase

   Tags

   SmBit

   Expression

   Mutation

   K30A

   Promoter

   HSV-TK

   Available Since

   Sept. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYTSK65K_8G7

   Plasmid

   #177303

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and GUS

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   uidA
   

   Use

   Synthetic Biology; Yeast expression, tn7 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYTRW14K_7G5

   Plasmid

   #177283

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and LacZ

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   lacZ
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MCP-Rep-X-RT∆R

   Plasmid

   #239384

   Purpose

   For circular RNA-mediated inverse prime editor using MCP-Rep-X-M MLV-RT∆RNase H in HEK294T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

   Insert

   MCP, Rep-X, M-MLV-RT(∆RNase H)
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ∆RNase H in M-MLV RT

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

   Plasmid

   #214950

   Purpose

   Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
   VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
   

   Use

   Tags

   6xHis, Amino half of venus BIFC fragment, Carboxy…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKT-CNP-inact

   Plasmid

   #211804

   Purpose

   Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

   Depositor

   Emily Weinert

   Article

   Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

   Insert

   2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H73L H153L

   Promoter

   tet

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKT-CNP

   Plasmid

   #211803

   Purpose

   Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

   Depositor

   Emily Weinert

   Article

   Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

   Insert

   2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p202-Syt1-C2A-I248V

   Plasmid

   #201554

   Purpose

   Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Synaptotagmin-1 C2A domain (partial)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Isoleucine 248 to Valine (numbering based…

   Promoter

   RBS

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet28b_His_RAD23A-UBA1

   Plasmid

   #201549

   Purpose

   Expresses UBA1 of human RAD23A in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Expresses amino acids 160-205 of DNA polymerase i…

   Promoter

   T7

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1BR9-AtWUSSTOP-Δα3

    Plasmid

    #202056

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    third helix of homeodomain deleted (AA82-102)

    Promoter

    T7 Promoter

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDR111_mannose_Stat1Klf6

    Plasmid

    #188400

    Purpose

    Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Inserts

    Stat-1 (Stat1 Mouse, Escherichia coli)
    Klf6 (Klf6 Mouse)
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET303-ColE1-TR-E192C-del1-40

    Plasmid

    #180235

    Purpose

    Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T and R domains (r…

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

    Plasmid

    #186414

    Purpose

    AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli)
    

    Use

    Gateway destination vector

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    Basal promoter of the FOG gene

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCB’ CsoS2 ΔNTD

    Plasmid

    #162705

    Purpose

    Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deletion of residues 2-256 of CsoS2, Y131D

    Promoter

    PLtet0-1 promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPPC002

    Plasmid

    #171139

    Purpose

    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    J1-BBa_J23117 and Sp.pCas9, BBa_J23107

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCCM’ CbbQ-

    Plasmid

    #162711

    Purpose

    Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Inactivation of CbbQ subunit of CbbOQ rubisco act…

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1A Prk-

    Plasmid

    #162694

    Purpose

    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Prk inactive (K20M S21A native protein, this map …

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBB0405-TEV-His12

    Plasmid

    #137040

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRevA-TEV-His12

    Plasmid

    #137034

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pP13-TEV-His12

    Plasmid

    #137035

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Human INPP5A

    Plasmid

    #129587

    Purpose

    Expression of human INPP5A in bacteria

    Depositor

    Christophe Erneux

    Article

    Erneux et al Eur J Biochem. 1995 Dec 1;234(2):598-602

    Insert

    Type I inositol 5-phosphatase (INPP5A Human)
    

    Use

    Tags

    Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZ_P-ICL1-LacI-Mit1AD

    Plasmid

    #126721

    Purpose

    LacI-Mit1AD on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Mit1 activation domain coding sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZ_P-ICL1-LacI-VP16

    Plasmid

    #126723

    Purpose

    LacI-VP16 on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-VP16 activation domain coding sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZ_P-ICL1-LacI-Gal4AD

    Plasmid

    #126724

    Purpose

    LacI-Gal4AD on pPICZ B expression vector

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    hybrid lacI-Gal4 activation domain coding sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pK_P-GAP-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126745

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pK_P-ICL1-LM_lacO-cP-AOX1-sAR

    Plasmid

    #126744

    Purpose

    yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    slovA,cpr
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    ICL1

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

    Plasmid

    #122450

    Purpose

    Expression of inositol 1,4,5-trisphosphate 3-kinase A

    Depositor

    Christophe Erneux

    Article

    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

    Insert

    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    

    Use

    Tags

    beta-galactosidase

    Expression

    Bacterial

    Mutation

    coding sequence from V66 till stop codon

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid

    #118269

    Purpose

    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

    Promoter

    T7 Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500

    Plasmid

    #92315

    Purpose

    Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    B1 domain of protein L (PL) mutant K16TAG
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70b (p70a promoter with one mutation)

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500

    Plasmid

    #92227

    Purpose

    Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP Y35TAG D193TAG
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues Y35 D193 were mutated into TAG (Amber) s…

    Promoter

    p70a

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid

    #92319

    Purpose

    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid

    #92318

    Purpose

    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92229

    Purpose

    Expression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70b (p70a promoter with one mutation)

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid

    #92230

    Purpose

    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residue V86 mutated into TAG (Amber) stop codon f…

    Promoter

    p70a

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZS1[GalS-T]

    Plasmid

    #60753

    Purpose

    Contains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    GalS-T
    GalS-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Chimeric LacI/GalR repressor with the TAN DBD and…

    Promoter

    Available Since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDM10

    Plasmid

    #41759

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc E. coli, Fly)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C45S C338S A378C C451S

    Promoter

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDM8

    Plasmid

    #41758

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc E. coli, Fly)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C45S C338S S364C C451S

    Promoter

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHyperTEV60

    Plasmid

    #228496

    Purpose

    10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    TEV Protease
    

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOpen-DpnIR

    Plasmid

    #165529

    Purpose

    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    DpnI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pSm-Nuclease

    Plasmid

    #132431

    Purpose

    Expresses Serratia marcescens nuclease in E. coli using T7 promoter

    Depositor

    Mark Arbing

    Insert

    Serratia marcescens nuclease (codon optimized)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTRC-LIC

    Plasmid

    #62343

    Purpose

    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter from pKK233-2

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFBOH-MHL

    Plasmid

    #62304

    Purpose

    It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    6xHis and TEV cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. YopH-pNIC-Bsa4

    Plasmid

    #92063

    Purpose

    pNIC expression venctor encoding gene for YopH

    Depositor

    Josh Beckham

    Insert

    YopH
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Codon optimized E. coli

    Promoter

    Available Since

    Jan. 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. p28BIOH-LIC

    Plasmid

    #62352

    Purpose

    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMalAnPL

    Plasmid

    #67281

    Purpose

    Expresses MBP-Anacystis nidulans photolyase in E. coli

    Depositor

    Aziz Sancar

    Insert

    photolyase Anacystis nidulans
    

    Use

    Tags

    maltose binding protein

    Expression

    Bacterial

    Mutation

    none known

    Promoter

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGEX4T1-NDFIP2

    Plasmid

    #179330

    Purpose

    E. coli optimized expression of GST-fusion of human NDFIP2

    Depositor

    Arthur Haas

    Insert

    NDFIP2 (NDFIP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Sc Rad27-pET24

    Plasmid

    #239204

    Purpose

    Overexpress Sc Rad27 (FEN1) in E. coli

    Depositor

    Michael O'Donnell

    Insert

    Rad27 (RAD27 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET/His-Rab1a(Q70L)

    Plasmid

    #232919

    Purpose

    E. coli Expression

    Depositor

    James Hurley

    Insert

    RAB1A (RAB1A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q70L

    Promoter

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAIP4_2RH3

    Plasmid

    #234219

    Purpose

    Heterologous protein expression of protein virC2 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2RH3 (virC2 Agrobacterium tumefaciens)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge