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pJWK_VD_02
Plasmid#158103Purpose5'UTR stem-loop luciferase reporter: -30kcal/molDepositorInsert5'UTR synthetic stem-loop -30kcal/mol
UseLuciferaseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(deltaN)
Plasmid#110189PurposeExpression of mouse MeCP2 (deltaN) in mammalian cellsDepositorInsertMeCP2_e2 delta N (mouse cDNA) _EGFP (Mecp2 Mouse)
UseTagsEGFPExpressionMammalianMutationdelta N terminusPromoterCMVAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Dronpa-FHA1
Plasmid#87711PurposeEncodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).DepositorInsertDronpa-FHA1
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE-cadherin-GFP-N-WASP deltaVCA
Plasmid#101283PurposeMammalian expression of E-cadherin-GFP-N-WASP delta VCADepositorUseTagsGFPExpressionMammalianMutationdelta VCA domainPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-NanoLuc-VChR1-EYFP
Plasmid#114112Purposefusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertNanoLuc; Volvox channelrhodopsin 1; EYFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374delNLS2
Plasmid#62532PurposeN-terminal aa1-269 and nuclear localization signal 2 of Drosha are deletedDepositorInserttruncated human Drosha mutant (DROSHA Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-269 and nuclear localizatio…PromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-OiRS3GG
Plasmid#98589PurposeExpresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertsgroup I intron
PheS cassette to be selectively replaced by unique asRNA probes
UseTagsExpressionBacterialMutationPromoterpBAD and pLtetOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP T271E
Plasmid#73057PurposeC-terminal GFP fusion of Mouse Foxg1 T271EDepositorInsertFoxg1 (Foxg1 Mouse)
UseTagsGFPExpressionMammalianMutationT271EPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG mFoxg1-GFP T271A
Plasmid#73047PurposeC-terminal fusion of gfp Mouse Foxg1 T271ADepositorInsertFoxg1 (Foxg1 Mouse)
UseTagsGFPExpressionMammalianMutationT271APromoterCAGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIS1-mutant RhoA 3'UTR
Plasmid#26090DepositorInsertmutant RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationBoth miR-31 binding sites in 3’ UTR mutagenized. …PromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-20nmEML-mRFP
Plasmid#231178PurposeStabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFPDepositorInsert20nmEML-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-30nmEML-mRFP
Plasmid#231179PurposeStabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFPDepositorInsert30nmEML-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-15nmEML-mRFP
Plasmid#231177PurposeStabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFPDepositorInsert15nmEML-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-FL_WobbleMut
Plasmid#209053PurposeGateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TAF5-∆ex8_WobbleMut
Plasmid#209054PurposeGateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3R/MPL-shRNA4
Plasmid#204529PurposeLentiviral expression of MPL-shRNA4; gene knock down of human MPLDepositorInsertMPL-shRNA4 (MPL Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3R/MPL-shRNA3
Plasmid#204528PurposeLentiviral expression of MPL-shRNA3; gene knock down of human MPLDepositorInsertMPL-shRNA3 (MPL Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3R/MPL-shRNA2
Plasmid#204527PurposeLentiviral expression of MPL-shRNA2; gene knock down of human MPLDepositorInsertMPL-shRNA2 (MPL Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
