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Showing: 1901 - 1920 of 21634 results

  2. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

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  8. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

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  9. pAGM22082_cRed

    Plasmid
    #117225
    Purpose
    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.
    Depositor
    Sylvestre Marillonnet
    Article
    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10
    Insert
    Canthaxanthin Operon
    Use
    Tags
    34 amino acid N-terminal linker, Hexahistidine Ta…
    Expression
    Bacterial
    Mutation
    removed Type IIS restriction sites within the bac…
    Promoter
    T7 RNA Polymerase
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    117225-plasmid-map-sequence-id-226673

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  13. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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Showing: 1901 - 1920 of 21634 results