Skip to main content

More Filters

We narrowed to 18,622 results for: Met

Showing: 4801 - 4850 of 18622 results
  1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129048
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129041
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

    Plasmid
    #129047
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA7 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

    Plasmid
    #129049
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA9 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129050
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA10 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129051
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA11 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

    Plasmid
    #129052
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA12 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129043
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA3 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129044
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA4 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

    Plasmid
    #129045
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA5 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

    Plasmid
    #129046
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA6 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
  15. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid
    #192786
    Purpose
    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNGR1 (IFNGR1 Human)
    Tags
    Ig k-chain leader sequence and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pSems-leader-mXFPe-IFNGR2(30-337)

    Plasmid
    #192787
    Purpose
    Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNGR2 (IFNGR2 Human)
    Tags
    Ig k-chain leader sequence and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. Capping Protein

    Plasmid
    #89950
    Purpose
    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.
    Inserts
    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    Tags
    6*His
    Expression
    Bacterial
    Mutation
    V222I
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
  18. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid
    #192785
    Purpose
    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. GEST-COMP-blac-flag-his

    Plasmid
    #111015
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Insert
    gamete egress and sporozoite traversal protein (GEST) (PF3D7_1449000 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  23. G protein alpha-s-YFP

    Plasmid
    #55781
    Purpose
    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Insert
    alpha-s-EE YFP (Gnas Rat)
    Tags
    EYFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    Met was substituted for Gln69 in EYFP (Clontech).…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  24. pSicoR Dnmt1

    Plasmid
    #12166
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF Dnmt1 shRNA expression.
    Depositor
    Insert
    Dnmt1 shRNA (Dnmt1 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    June 30, 2006
    Availability
    Academic Institutions and Nonprofits only
  25. pGLU_113

    Plasmid
    #225794
    Purpose
    RS421 library variant 13453
    Depositor
    Insert
    13453_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. pGLU_95

    Plasmid
    #225809
    Purpose
    RS421 library variant 13453
    Depositor
    Insert
    13453_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  27. pGLU_77

    Plasmid
    #225779
    Purpose
    RS421 library variant 13453
    Depositor
    Insert
    13453_[abr]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  28. FRP935

    Plasmid
    #159302
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection
    Depositor
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  29. MT1K

    Plasmid
    #105707
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1k
    Depositor
    Insert
    Metallothionein 1k
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  30. pIAneo

    Plasmid
    #52118
    Purpose
    a bicistronic IRES-mAmetrine 1.1 containing vector for mammalian expression (CMV promoter)
    Depositor
    Type
    Empty backbone
    Tags
    IRES-mAmetrine 1.1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  31. pGEX-CS-MjPiwi_G

    Plasmid
    #146384
    Purpose
    Bacterial Expression of MjPiwi
    Depositor
    Insert
    MjPiwi
    Expression
    Bacterial
    Mutation
    one missense mutation C490A (H163N) compared to t…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  32. MT1G

    Plasmid
    #105704
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1g
    Depositor
    Insert
    Metallothionein 1g (MT1G Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  33. MT1X

    Plasmid
    #105709
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1x
    Depositor
    Insert
    Metallothionein 1x (MT1X Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  34. MT1F

    Plasmid
    #105703
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1f
    Depositor
    Insert
    Metallothionein 1f (MT1F Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  35. pPD301 ZF43x16-Compact_EYFP

    Plasmid
    #138911
    Purpose
    EYFP reporter vector for the ZF43x16-Compact promoter
    Depositor
    Insert
    ZF43x16-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x16-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  36. pPD299 ZF43x14-Compact_EYFP

    Plasmid
    #138909
    Purpose
    EYFP reporter vector for the ZF43x14-Compact promoter
    Depositor
    Insert
    ZF43x14-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x14-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. pPD294 ZF43x10-Compact_EYFP

    Plasmid
    #138905
    Purpose
    EYFP reporter vector for the ZF43x10-Compact promoter
    Depositor
    Insert
    ZF43x10-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x10-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  38. pPD300 ZF43x15-Compact_EYFP

    Plasmid
    #138910
    Purpose
    EYFP reporter vector for the ZF43x15-Compact promoter
    Depositor
    Insert
    ZF43x15-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x15-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  39. pPD298 ZF43x13-Compact_EYFP

    Plasmid
    #138908
    Purpose
    EYFP reporter vector for the ZF43x13-Compact promoter
    Depositor
    Insert
    ZF43x13-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x13-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  40. pPD295 ZF43x11-Compact_EYFP

    Plasmid
    #138906
    Purpose
    EYFP reporter vector for the ZF43x11-Compact promoter
    Depositor
    Insert
    ZF43x11-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x11-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  41. pPD293 ZF43x9-Compact_EYFP

    Plasmid
    #138904
    Purpose
    EYFP reporter vector for the ZF43x9-Compact promoter
    Depositor
    Insert
    ZF43x9-Compact
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    ZF43x9-Compact
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 4801 - 4850 of 18622 results