We narrowed to 15,732 results for: LEA

1. cASIC1 concatemer - Subunit A

   Plasmid

   #233129

   Purpose

   To create cASIC1 channels of defined stoichiometry in mammalian cells

   Depositor

   David MacLean

   Article

   Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

   Insert

   ASIC1 (ASIC1 Chicken)
   

   Expression

   Mammalian

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTarget-Dual

   Plasmid

   #207884

   Purpose

   Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

   Depositor

   David Edgell

   Article

   Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

   Inserts

   I-OnuI target site
   I-GpeMI target site
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. M-NylC_pET28a

   Plasmid

   #215409

   Purpose

   Expression construct for M-NylC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   M-NylC
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP8GF-ZmAFB2/3 b2

   Plasmid

   #188425

   Purpose

   Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   Auxin Signaling Receptor AFB2/3 b2
   

   Expression

   Yeast

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP8GF-ZmAFB2/3 a

   Plasmid

   #188423

   Purpose

   Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   Auxin Signaling Receptor AFB2/3 a
   

   Expression

   Yeast

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP4G-REL2N91-ccdB

   Plasmid

   #188444

   Purpose

   Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   REL2N91/ccdB
   

   Expression

   Yeast

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-ZmIAA8ΔPB1

   Plasmid

   #188432

   Purpose

   Zea Mays Nuclear Auxin Signaling Repressor IAA8

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   Auxin Signaling Repressor IAA8deltaPB1
   

   Use

   Cloning

   Mutation

   deleted PB1 domain

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. KBB441

   Plasmid

   #185131

   Purpose

   Swi6 full length with L->A mutated in nuclear localization signal

   Depositor

   Ken Belanger

   Article

   Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

   Insert

   SWI6
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   SWI6 deletion of amino acids 450-458

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ppwd1.473.646.W601A

   Plasmid

   #137660

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Melissa Jurica, Tara Davis

   Article

   Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

   Insert

   ppwd1 (PPWD1 Human)
   

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   473-646-W601A

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD44I-c014

    Plasmid

    #175451

    Purpose

    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    AviTag-His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD44U-c011

    Plasmid

    #175454

    Purpose

    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    AviTag-His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CD44I-c011

    Plasmid

    #175449

    Purpose

    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKI-cjOCT4-Cyan-pac

    Plasmid

    #160871

    Purpose

    Marmoset Targeting

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

    Insert

    mCerulean3
    

    Use

    Marmoset targeting

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBW-m4046782

    Plasmid

    #145630

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGBW-m4046821

    Plasmid

    #145631

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 leader protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGBW-m4046857

    Plasmid

    #145585

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGBW-m4046902

    Plasmid

    #145587

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCAG-T3-HypaCAS9-pA

    Plasmid

    #131467

    Purpose

    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

    Insert

    Codon optimized HypaCas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM0652

    Plasmid

    #129013

    Purpose

    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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