19,624 results

1. pGEX-KG-D4H*-mCherry

   Plasmid

   #134604

   Purpose

   fluorescent cholesterol-binding probe

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   D4H*
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   Y415A and A463W

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEv-Fat4-citrine

   Plasmid

   #112854

   Purpose

   Constitutive expression of human fat4 protein fused to citrine fluorescent protein

   Depositor

   David Sprinzak

   Article

   Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

   Insert

   FAT4 (FAT4 Human)
   

   Tags

   Citrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-JAK2

   Plasmid

   #23915

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1-4EBP1_4xAla

   Plasmid

   #38240

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Mutation

   T37A, T46A, S65A, T70A (dominant negative)

   Promoter

   Tet ON

   Available Since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAX APP 751

   Plasmid

   #30138

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Expression

   Mammalian

   Mutation

   wild type splice variant 751

   Available Since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

   Plasmid

   #103865

   Purpose

   Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Contains the H133A and H1058A mutations which ina…

   Promoter

   EF1a

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK7-HA-S6K1-WT

   Plasmid

   #8984

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. hAAVS1 1R TALEN

   Plasmid

   #35432

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1R TALEN
   

   Expression

   Mammalian

   Available Since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 GST TEV LIC cloning vector (1G)

   Plasmid

   #29655

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. hAAVS1 1L TALEN

    Plasmid

    #35431

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1L TALEN
    

    Expression

    Mammalian

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #46295

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    PSD95.FingR-eGFP-CCR5TC (DLG4 Human, Synthetic)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG1K

    Plasmid

    #71741

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Bacterial

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. plRL117

    Plasmid

    #163635

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available Since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRK5-myc-Miro1

    Plasmid

    #47888

    Purpose

    Expresses myc tagged Miro1

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hsyn-ASAP5-WPRE

    Plasmid

    #225709

    Purpose

    Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5
    

    Use

    AAV

    Promoter

    human synapsin

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. E-cadherin-GFP

    Plasmid

    #28009

    Depositor

    Jennifer Stow

    Article

    Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

    Insert

    E-cadherin (CDH1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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