We narrowed to 11,958 results for: pcDNA

1. pcDNA5-FRT/TO-eGFP-53BP1

   Plasmid

   #60813

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA3.2 V5-DEST 3XFlag-SRSF3

   Plasmid

   #46736

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   SRSF3 (SRSF3 Human)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-puro-eGFP-HEC1

   Plasmid

   #114022

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA pDEST40 Cas9-POLD3

   Plasmid

   #183194

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   POLD3
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA6.2 EmGFP hAQP4(M23)

   Plasmid

   #126464

   Purpose

   Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

   Insert

   Aquaporin 4 (AQP4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Flag-mRIPK1-S321A

   Plasmid

   #115339

   Purpose

   Mammalian expression of Flag-mRIPK1-S321A

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321A

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 EYFP H148Q/I152L

   Plasmid

   #25872

   Depositor

   Peter Haggie

   Article

   Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

   Insert

   EYFP H148Q/I152L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP from pEYFP (Clontech) with H148Q and I152L m…

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3.1-pCMV-PfV-GS-Sapphire

   Plasmid

   #116933

   Purpose

   Mammalian vector for expression of the 40nm GEM

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Tags

   Sapphire

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Cyto-ExRai-AktAR2

    Plasmid

    #184048

    Purpose

    Cytosol-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Cyto-ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+) CircRNA Mini Vector

    Plasmid

    #60648

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3-FLAG-human NPM1 wt

    Plasmid

    #160692

    Purpose

    Mammalian expression of FLAG-tagged NPM1

    Depositor

    Charles Sherr

    Article

    Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT_mEGFP-SNAP-tag2

    Plasmid

    #226513

    Purpose

    Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    mEGFP-SNAP-tag2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Use

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3puro-mGFP-FXR1-isoform a

    Plasmid

    #225447

    Purpose

    Express EGFP-fusion protein of FXR1 isoform a

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-SARS-CoV-2-S (Wuhan)

    Plasmid

    #215812

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71294

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    GNSTM glycosylation motif, HA, Lamp2 signal pepti…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA5/FRT_CMV_NES-Kinprola_JNK-mEGFP

    Plasmid

    #233359

    Purpose

    CMV driven expression of the JNK activity recorder Kinprola_JNK in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_JNK-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT_CMV_NES-Kinprola_AMPK-mEGFP

    Plasmid

    #233361

    Purpose

    CMV driven expression of the AMPK activity recorder Kinprola_AMPK in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_AMPK-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA5/FRT_CMV_NES-Kinprola_PKC-mEGFP

    Plasmid

    #233357

    Purpose

    CMV driven expression of the PKC activity recorder Kinprola_PKC in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKC-mEGFP
    

    Use

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid

    #71293

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-PB1-B/BRISBANE/60/2008

    Plasmid

    #187919

    Purpose

    Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Use

    Tags

    UNTAGGED

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-Nuc-ExRai-AktAR2

    Plasmid

    #184049

    Purpose

    Nuclear-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Nuc-ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA 3.2/V5-DEST CDC42EP1 WT

    Plasmid

    #69819

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA GASTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71295

    Purpose

    Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    GASTM mutated glycosylation motif, HA, Lamp2 sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 Flag-HIF1B (#930)

    Plasmid

    #99916

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

    Insert

    HIF1B (ARNT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Promoter

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3 LIC cloning vector (6A)

    Plasmid

    #30124

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3 2xFLAG-2xSTREP E2F7

    Plasmid

    #236442

    Purpose

    transient overexpression of E2F7 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-hAsCpf1(TATV) (pY220)

    Plasmid

    #89353

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TATV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 1036 pcDNA3 Myr HA Akt1

    Plasmid

    #9008

    Purpose

    Mammalian expression of human Akt1 with myr, HA tags

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1 ZoAl-ENT N-1F (R1103A)

    Plasmid

    #216548

    Purpose

    For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-HIF1alpha P402A/P564A-pcDNA3

    Plasmid

    #18955

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 iCasper T2A HO1

    Plasmid

    #64278

    Purpose

    Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge