2,159 results

1. pBT2-4xUAS:NICD-P2A-mRFP

   Plasmid

   #127594

   Purpose

   Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Insert

   NICD (notch1a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-Opto-zFGFR1a

   Plasmid

   #232639

   Purpose

   Zebrafish FGFR1a receptor kinase domain fused to VfLOV

   Depositor

   Katherine Rogers

   Insert

   zFGFR1a kinase domain + VfLOV domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. bactin2:loxP-STOP-loxP-DsRed-express

   Plasmid

   #68797

   Purpose

   For making transgenic fish

   Depositor

   David Traver

   Article

   Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

   Inserts

   beta-actin promoter
   loxp-stop-codon-loxp-dsred
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

   Plasmid

   #24334

   Depositor

   Didier Stainier

   Article

   Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

   Insert

   H2B
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   mCherry driven by an HSP70 promoterCerulean drive…

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR5'_ubi:loxP-EGFP-loxP

   Plasmid

   #27322

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   ubiquitin:loxP-EGFP-loxP
   

   Use

   Multisite gateway 5' entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-mfap4

   Plasmid

   #70052

   Purpose

   5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

   Depositor

   David Tobin

   Article

   Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

   Insert

   mfap4 promoter
   

   Use

   Gateway 5' element

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Phat2-dr-cPla2-C2-EGFP-6H

   Plasmid

   #187001

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSDARG00000024546 (pla2g4aa Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

   Plasmid

   #187186

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSDARG00000024546 (pla2g4aa Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Y90F, V91F

   Promoter

   Available since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MiniCoopR mitfa:EGFP

   Plasmid

   #118850

   Purpose

   Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   mitfa: EGFP (mitfa Synthetic, Zebrafish)
   

   Use

   Zebrafish expression, tol6

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E-mitfa2.1

    Plasmid

    #81234

    Purpose

    zebrafish mitfa promoter entry vector for multisite Gateway cloning

    Depositor

    James Lister

    Insert

    mitfa promoter (mitfa Zebrafish)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    mitfa, 2.1kb including 5' UTR

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Rab13 in pCSDEST2

    Plasmid

    #140879

    Purpose

    Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab13 (rab13 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tg(mpeg1:LifeAct-mRuby)

    Plasmid

    #166977

    Purpose

    labels F-actin in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

    Inserts

    mpeg1 (mpeg1.1 Zebrafish)
    LifeAct (actb1 Frog)
    

    Use

    Tags

    Lifeact-mRuby and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid

    #105648

    Purpose

    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

    Depositor

    Sandra Rieger

    Insert

    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2FA CO Tol2 TPase

    Plasmid

    #133032

    Purpose

    Expression vector for production of codon optimized Tol2 transposase

    Depositor

    Charles Nathan Hancock

    Article

    Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

    Insert

    Codon optimized Tol2 Transposase
    

    Use

    In vitro expression

    Tags

    Expression

    Mutation

    Codon Optimized

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dat - EGFP - Tol2

    Plasmid

    #85418

    Purpose

    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

    Depositor

    Marc Ekker

    Article

    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

    Insert

    dopamine transporter (slc6a3 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    dat

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pDestTol2-actinb-kcna1a-IRES-EGFP

    Plasmid

    #164965

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcna1a (kcna1a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-zTRAP

    Plasmid

    #42236

    Depositor

    Stephen L Johnson

    Article

    Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

    Insert

    EGFP-Rpl10a (rpl10a Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5E-mpeg1.1

    Plasmid

    #75023

    Purpose

    Can be used to drive expression in zebrafish microglia.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    mpeg1.1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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