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Showing: 1 - 20 of 46 results
  1. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid
    #133319
    Purpose
    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    vanillate
    Availability
    Academic Institutions and Nonprofits only
  2. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Availability
    Academic Institutions and Nonprofits only
  3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid
    #133339
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus
    Depositor
    Insert
    sgRNA_ctrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
  4. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid
    #133340
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
    Depositor
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
  5. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

    Plasmid
    #133341
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus
    Depositor
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
  6. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133344
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus
    Depositor
    Insert
    sgRNA_cpaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
  7. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133345
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus
    Depositor
    Insert
    sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Availability
    Academic Institutions and Nonprofits only
  8. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Availability
    Academic Institutions and Nonprofits only
  9. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Availability
    Academic Institutions and Nonprofits only
  10. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Availability
    Academic Institutions and Nonprofits only
  11. U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

    Plasmid
    #136651
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.
    Depositor
    Inserts
    St1Cas9 LMD-9:CNRZ_1066 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Availability
    Academic Institutions and Nonprofits only
  12. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 46 results