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Addgene
Showing: 61 - 80 of 93 results

  5. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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  6. pAAV-SCP1-dSa-VPR mini.

    Plasmid
    #99685
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99685-plasmid-map-sequence-id-194598

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  7. pAAV-EFS-dSa-VPR mini.

    Plasmid
    #99686
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99686-plasmid-map-sequence-id-194608

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  9. pAAV-CMV-dSa-VPR mini.-1X snRP1

    Plasmid
    #99687
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99687-plasmid-map-sequence-id-194615

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  10. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid
    #99689
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99689-plasmid-map-sequence-id-194506

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  11. pAAV-CMV-dSa-VPR mini.-2X snRP1

    Plasmid
    #99688
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99688-plasmid-map-sequence-id-194622

    Enlarge

  12. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

    Enlarge

  13. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid
    #99696
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Afp (Afp Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99696-plasmid-map-sequence-id-194593

    Enlarge

  14. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Actc1 (Actc1 Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

    Enlarge

  15. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Actc1 (Actc1 Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

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  18. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Expression
    Mutation
    Promoter
    PgpdA and PtrpC
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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Showing: 61 - 80 of 93 results