Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 21 - 40 of 128241 results

  10. pINSPECT_SigP:NLuc

    Plasmid
    #197263
    Purpose
    Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197263-plasmid-map-sequence-id-389724

    Enlarge

  11. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

    Enlarge

  13. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

    Plasmid
    #173213
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Availability
    Academic Institutions and Nonprofits only
    173213-plasmid-map-sequence-id-339522

    Enlarge

  17. GBH-R-insBFP.1

    Plasmid
    #188007
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Availability
    Academic Institutions and Nonprofits only
    188007-plasmid-map-sequence-id-380746

    Enlarge

  18. GBH-R-insBFP.2

    Plasmid
    #188008
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Availability
    Academic Institutions and Nonprofits only
    188008-plasmid-map-sequence-id-381105

    Enlarge

  19. GBH-R-insBFP.3

    Plasmid
    #188009
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin BFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Availability
    Academic Institutions and Nonprofits only
    188009-plasmid-map-sequence-id-381106

    Enlarge

  20. GBH-R-insGFP.1

    Plasmid
    #188010
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker insulin GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; insulin promotor
    Availability
    Academic Institutions and Nonprofits only
    188010-plasmid-map-sequence-id-381122

    Enlarge

Showing: 21 - 40 of 128241 results