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pTV-Klf4-tdiRFP670-PEST

Plasmid

#122350

Purpose

Targeting vector for mouse Klf4 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Klf4

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-329

Plasmid

#140146

Purpose

GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 Kruppel factor (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 330 to 479

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-156

Plasmid

#140147

Purpose

GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 1-156 (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 157 to 479

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KLF4

Plasmid

#192908

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

KLF4 (KLF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2-17-p2a-KLF4

Plasmid

#193290

Purpose

episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2AV-p2a-KLF4

Plasmid

#193291

Purpose

episomal expressing human SOX2A61V + KLF4

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2AV-p2a-KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2-p2a-KLF4

Plasmid

#193292

Purpose

episomal hSK (expressing human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-p2a-KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

F2A of original pCXLE-SK vector was replaced with…

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-Report-Luc-KLF4-K1S

Plasmid

#62979

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 1-446 of KLF4

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K2S

Plasmid

#62980

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 446-911 of KLF4

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K3S

Plasmid

#62981

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 911-1406 of KLF4

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K4S

Plasmid

#62982

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 1406-1781 of KLF4

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K6S

Plasmid

#62983

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 2086-2639 of KLF4

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S

Plasmid

#34598

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Fragment 1781-2086 of KLF4

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
EF1a-3NLS-Klf4 TALE-GCN4

Plasmid

#120546

Purpose

Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Availability

Academic Institutions and Nonprofits only

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Empty Bridge Control Zfp462-Klf4SE

Plasmid

#127666

Purpose

Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

gRNAs 129, 135, 115, 117

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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LADL Bridge + Target Zfp462-Klf4SE

Plasmid

#127668

Purpose

Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a and hU6

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL GAL4dbd-KLF4 159-329

Plasmid

#140148

Purpose

GAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 159-329 (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 1 to 158 and 330 to 479

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-Klf4-pA-pgk-hph

Plasmid

#74906

Purpose

Mammalian expression of mKlf4

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Klf4 (Klf4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17

Plasmid

#193299

Purpose

tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Klf4-IRES-Sox2-17 (Sox2, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Availability

Academic Institutions and Nonprofits only

Enlarge