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We narrowed to 172 results for: pef gfp

Showing: 1 - 20 of 172 results
  1. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid
    #71719
    Purpose
    Expressing a GFP-tagged degron in the soma of C. elegans
    Depositor
    Insert
    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    Tags
    EmGFP
    Expression
    Worm
    Mutation
    minimal degron sequence (71-114aa) with start cod…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  2. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pEF5B-FRT-control-APEX

    Plasmid
    #73187
    Purpose
    Expresses "control"-APEX (cytosolic) in mammalian cells
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    control-APEX (Nphp3 Glycine max, Mouse)
    Tags
    internal GFP tag
    Expression
    Mammalian
    Mutation
    changed glycine 2 to alanine
    Promoter
    EF1alpha
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid
    #100941
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag
    Depositor
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Human, Synthetic)
    Tags
    1xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid
    #49098
    Purpose
    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sstr3 (Sstr3 Mouse)
    Tags
    GFP and acceptor peptide (AP)
    Expression
    Mammalian
    Promoter
    T7
    Available since
    Nov. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  7. 5HRE/GFP

    Plasmid
    #46926
    Purpose
    hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5X HRE of VEGF (VEGFA Human)
    Tags
    d2EGFP
    Expression
    Mammalian
    Mutation
    five copies of a 35-bp fragment from the hypoxia-…
    Promoter
    EF-1α promoter
    Available since
    Aug. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 1 - 20 of 172 results