Skip to main content
Addgene
Showing: 1 - 20 of 355 results

  3. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193314
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193314-plasmid-map-sequence-id-382366

    Enlarge

  4. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

    Enlarge

  5. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193316
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193316-plasmid-map-sequence-id-382501

    Enlarge

  6. pLenti Puro HA-Ubiquitin-WT

    Plasmid
    #236289
    Purpose
    Lentiviral vector expressing HA-tagged wild type ubiquitin
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236289-plasmid-map-sequence-id-467836

    Enlarge

  7. pLenti Puro HA-Ubiquitin-KO

    Plasmid
    #236292
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin KO mutant
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin KO (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing KO mu…
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236292-plasmid-map-sequence-id-468935

    Enlarge

  8. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193318
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193318-plasmid-map-sequence-id-383635

    Enlarge

  9. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193317
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    193317-plasmid-map-sequence-id-383630

    Enlarge

  10. pLenti Puro HA-Ubiquitin-K48

    Plasmid
    #236290
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin K48 mutant
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin K48 (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing K48 m…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236290-plasmid-map-sequence-id-467837

    Enlarge

  11. pLenti Puro HA-Ubiquitin-K63

    Plasmid
    #236291
    Purpose
    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant
    Depositor
    Addgene Research Program, Melina Fan
    Insert
    Ubiquitin K63 (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Single ubiquitin coding sequence containing K63 m…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236291-plasmid-map-sequence-id-467835

    Enlarge

  20. pLSU1/Os-ubi:nnLuz-I{PIV-216}:NosT

    Plasmid
    #212191
    Purpose
    This binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the rice ubiquitin (R. ubi) promoter and UTR
    Depositor
    Wayne Curtis
    Insert
    nnLuz-I{PIV-216}
    Use
    Tags
    Expression
    Plant
    Mutation
    PIV intron added at bp 216, eliminates expression…
    Promoter
    Rice ubiquitin
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212191-plasmid-map-sequence-id-443882

    Enlarge

Showing: 1 - 20 of 355 results