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Showing: 41 - 60 of 81 results

  3. pMS2M-PH-gagpol-D64V

    Plasmid
    #166031
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    MS2-Gag-Pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166031-plasmid-map-sequence-id-328290

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  4. pMDlg/pRRE-int-MS2M

    Plasmid
    #166032
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    Gag-Pol-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166032-plasmid-map-sequence-id-338747

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  6. px552-U6-gRNA1-CMV-eGFP

    Plasmid
    #211758
    Purpose
    sgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Glenn Yiu
    Insert
    gRNA1 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    211758-plasmid-map-sequence-id-421556

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  7. px552-U6-gRNA2-CMV-eGFP

    Plasmid
    #211759
    Purpose
    sgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Glenn Yiu
    Insert
    gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    211759-plasmid-map-sequence-id-421553

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  8. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid
    #211760
    Purpose
    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Glenn Yiu
    Insert
    gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    211760-plasmid-map-sequence-id-421239

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  9. pCSD_FRB-TALETS3-3xFLAG-2xNLS

    Plasmid
    #107306
    Purpose
    Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    TALE_VEGFA-TS3
    Use
    CRISPR
    Tags
    3x Flag, 2xNLS and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Availability
    Academic Institutions and Nonprofits only
    107306-plasmid-map-sequence-id-204787

    Enlarge

  11. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107303
    Purpose
    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS2
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Availability
    Academic Institutions and Nonprofits only
    107303-plasmid-map-sequence-id-204639

    Enlarge

  12. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107304
    Purpose
    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS3
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Availability
    Academic Institutions and Nonprofits only
    107304-plasmid-map-sequence-id-204643

    Enlarge

  16. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

    Enlarge

  19. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107299
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Availability
    Academic Institutions and Nonprofits only
    107299-plasmid-map-sequence-id-204615

    Enlarge

  20. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107300
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Availability
    Academic Institutions and Nonprofits only
    107300-plasmid-map-sequence-id-204631

    Enlarge

Showing: 41 - 60 of 81 results