Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 986 results for: igh

Showing: 1 - 50 of 986 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...LEU13 IGH@ immunoglobulin heavy locus DKFZp686C15213, IGH, IGH.1@, IGHDY1, MGC72071, MGC88774 IGHA1 immunoglobulin...diversity 7-27 DHQ52, IGHD727 IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy...variable 1-58 IGHV158, VH IGHV1-69 immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin...-30-3 IGHV3-3, IGHV3303 IGHV3-30-5 immunoglobulin heavy variable 3-30-5 IGHV3-3, IGHV3305 IGHV3-33 immunoglobulin...-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304 IGHV4-31 immunoglobulin...diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-functional) DM2, IGHD114 IGHD1-20 immunoglobulin...heavy diversity 1-20 IGHD120 IGHD1-26 immunoglobulin heavy diversity 1-26 IGHD126 IGHD1-7 immunoglobulin ...

  2. Brain Initiative Collection

    Type
    Collection
    ... our collection! AAV are distributed as high-quality and high-titer 100 µL solutions of purified viral...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper...AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin...AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance...transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017 Jun 29...Red genetically encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors ...to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage sensor to detect...organelle-localized). Browse or search the tables below highlighting a few popular categories of biosensors or browse...NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924...for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: ...

  4. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dLight1.1 Syn dLight1.1 none Constitutive 1 Tian 111067 pAAV-CAG-dLight1.1 CAG dLight1.1 none Constitutive...pAAV-hSyn-dLight1.2 Syn dLight1.2 none Constitutive 1, 5 Tian 125560 AAV-CAG-dLight1.3b CAG dLight1.3b none...pAAV-syn-dLight1.3b Syn dLight1.3b none Constitutive 9 Tian 125707 pAAV-CAG-RdLight1 CAG RdLight1 none Constitutive...Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors...187179 pAAV_hSyn1_nLightG Syn nLightG none Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR...Constitutive 9 Tian 125708 pAAV-hSynapsin1-RdLight1 Syn RdLight1 none Constitutive 9 Tian 113049 pAAV-hSyn-GRAB_DA1m...none Cre dependent 9 Tian 163909 pAAV_hSynapsin_psychLight2 Syn psychLight2 none Constitutive 9 Tian GABA...

  5. Antibody Guide

    Type
    Collection
    ...the Fab fragments contain highly variable regions, shown in light blue and light orange, that bind to a ...are light-sensitive molecules which emit a specific wavelength of light after excitation by light of a...pair the brightness of the fluorophore with the abundance of the antibody target. If a bright fluorophore...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...-efficient way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies...protein chains: two large heavy chains and two small light chains. These four domains come together to form... to be produced in a single individual, giving a high probability that an antibody will be able to recognize...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ... of interest. Tight TRE promoter or P Tight : reduced background expression and tighter binding of Tet-Advanced...gene of interest. tTA-Advanced Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled...systems and how to use them, or skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression... Left: natural TetR mechanism; center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled... level of doxycycline can access the brain so a highly sensitive transactivator may be needed. Or, when...effector for tet-responsive systems. Dox binds with high affinity to TetR and derivatives like rTetR, rtTA...know whether your serum is tetracycline-free. Highlighted Plasmids Browse the tables below to find popular...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Excitation and Emission maximum are listed in nm. Brightness is the product of exctinction coefficient and...low concentration but are prone to dimerization at high concentration, so this is noted here. Proteins that...Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 ...Top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine ...Top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids iLOV 447 497... Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527... Orange Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...Plasmids and Resources Plasmid Tools Collection Highlights Other Resources The zebrafish ( Danio rerio )...large populations that are amenable to genetics, high-throughput screening and non-invasive live imaging..., and drug discovery and development. Here we highlight some of the plasmids and tools available at Addgene... zebrafish gene or sequence . The table below highlights plasmids that contain tools for use in zebrafish...Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection CRISPR Please...control of gene expression in zebrafish using red light. LipoGlo - Steven Farber Lab. A reporter system ...lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab....

  9. CRISPR Guide

    Type
    Collection
    ...joining (NHEJ) pathway and the less efficient but high-fidelity homology directed repair (HDR) pathway....also enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no ..., which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum... downstream of the target site (termed left and right homology arms). The length of each homology arm ...available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...strands Prime editing and base editing both offer high efficiency and low indel formation compared to HDR...favor insertion in the T-RL direction, with the right homology end closest to the target site. Type V-...

  10. Bacterial Expression Systems

    Type
    Collection
    ...constitutively express your gene of interest at high levels. Perhaps high expression leads to slow bacterial growth...pREDawn-AmpR-MCS FixK2 Red light (660 nm) Escherichia coli Heikki Takala 31395 pJT118 PcpcG2 Green Light (532 nm) Escherichia...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the ...Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins... be controlled by a variety of small molecules, light, and temperature, in different bacterial systems...Escherichia coli Dominic Esposito 43796 pDawn FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940...coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... to control cell activity in living tissue with light. Optogenetics is typically used in neuroscience ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent...pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive ...Deisseroth 137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...

  12. Plan Your Experiment

    Type
    Collection
    ...for a given experiment. In the table below, we highlight the most common categories of genetic manipulations...joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity Dual-nickase...editors Editing efficiency can be low, but generally higher than HDR pegRNA optimization can be challenging...location. Positioning your chosen Cas enzyme at the right spot within the genome relies on proper gRNA design...design a donor DNA to have the appropriate left and right homology arms. See Addgene's HDR blog post for more...guides that target closer to the edit site yield higher editing efficiencies. The template sequences can...specificity, consider using an engineered Cas enzyme with higher fidelity. In addition to off-target activity, it...

  13. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 5 amino acid linker V17V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 17 amino acid linker V32V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 32 amino acid linker VVV Anisotropy/brightness standard consisting of three Venus fluorescent...amino acid linker, respectively VVVV Anisotropy/brightness standard consisting of four Venus fluorescent...amino acid linker, respectively VVVVV Anisotropy/brightness standard consisting of five Venus fluorescent...connected via 5 amino acid linkers VVVVVV Anisotropy/brightness standard consisting of six Venus fluorescent ...

  14. Antibody Plasmid Collection

    Type
    Collection
    .... In addition to being useful for our bodies to fight off intruders, antibodies are also extremely useful...to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use it to...and Expressing Antibodies The following table highlights plasmid collections that can be used to create...Article PI One-step assembly of antibody heavy- and light-chain DNAs into a single mammalian expression vector...Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv...Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...

  15. Promega Plasmid Collection

    Type
    Collection
    ...assays use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...19.1 kDa and exceptionally bright and stable luciferase that enables highly sensitive detection at endogenous...CRISPR-mediated gene editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc ...interest and monitoring endogenous protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link ...with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT antibody for additional detection...together to form an active enzyme and generate a bright luminescent signal in the presence of a substrate...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...collections for use in plants. The tables below highlight plasmids spanning a wide variety of molecular ...The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, ... Li. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and...Return to top Empty Backbones The table below highlights plasmids that can be used as empty backbones ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent...

  17. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ... nLightR. A, Structural models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita...indicators: nLightG and nLightR (Kagiampaki et al., 2023).     Figure 1: Properties of nLightG and nLightR...al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...norepinephrine. nLight is 28-fold more selective than GRABNE, with both nLightG and nLightR showing significantly...experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly expand the toolbox... both nLightG and nLightR, and may soon have a red tool which matches the sensitivity of nLightG. Many...images of HEK293T cells and neurons expressing nLightG or nLightR before/after application of NE (10 μM) and...

  18. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging...near or above 650 nm as signals below 650 nm are highly absorbed by hemoglobin and cause undesirable background...iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially selective imaging of ...Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal...Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1... Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation...

  19. Optogenetics Plasmid Collection

    Type
    Collection
    ...proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins...archaebacteria, that can be opened and closed in response to light. These proteins can be expressed in specific neurons...membrane channel that opens in response to blue light and allows cations to flow into the cell. Variants...cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as...or search the table below for plasmids that use light to control a protein or cellular activity. For example...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...Expression Constructs Reporter Constructs Other Highlights You may also like... Fluorescent Proteins Blog...and detection wavelengths. Firefly luciferase is brighter than Renilla luciferase, but is more susceptible... applications. NanoLuc® is small (19 kDa), very bright, and ATP-independent, and a secreted version of...mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc...Description Gene/Insert PI Return to Top Other Highlighted Tools Modified/Novel Luciferases Engineered variants...use the substrate Akalumine with significantly brighter signal and emission in the near-infrared range...

  21. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions ...vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin light chain Clathrin-coated...cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the ...Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions...-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 ...

  22. AAV Packaged on Request

    Type
    Collection
    ...includes MTA facilitation, DNA amplification, and high-quality quality control measures such as titering...offer 966 in-stock viral vector preps, delivering high quality and ready availability at affordable prices...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists...Viral Vector Production Learn about how we produce high-quality viral vector preps at Addgene, including...techniques (Link opens in a new window) for producing high-quality research-grade viral vector preps. Data ... that complement published data, capturing the insights that often go unshared. Help Center Check out ...

  23. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF,...95% of archaeal and 48% of bacterial genomes, are highly diverse, with varying PAM sequences and number/...) Type V (Cpf1) Type IV (Csf1) Type VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive...creating a double-strand break. This method displays higher efficiency than HDR-mediated editing. The first... Genome engineers continue to work to develop a highly specific, programmable platform well-suited for...MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable...

  24. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...and T492V. AAV44.9 The AAV44.9 serotype displays highly efficient transduction and lateral spread in the...Arun Srivastava and cite Petrs-Silva, et al. 2009. High-efficiency transduction of the mouse retina by tyrosine-mutant...a mouse model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39....acknowledge Arun Srivastava and cite Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction of Primary Human Hematopoietic...

  25. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre , other recombinases... iCre or FLPo in mammalian cells may facilitate higher rates of Cre-driven recombination. Gene Expression... leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below to find popular...search for "split", "rapamycin", “tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description...

  26. COVID-19 Resources

    Type
    Collection
    ... maintain this plasmid collection page, which highlights plasmids available that may be useful for COVID...called SARS-CoV-2. Scientists have been working at lightning speed to elucidate as much as they can about this... develop and optimize the method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK... now available from Xiaokun Shu's lab. A rapid, highly sensitive and open-access SARS-CoV-2 detection ...Transcriptase plasmids from Andrew Ellington's lab. High-surety isothermal amplification and detection of...

  27. Genetic Code Expansion

    Type
    Collection
    ...that it is responsive to specific stimuli, such as light. On a broader scale, the expanded genetic code can... . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA synthetase...TAG Edward Lemke Browse Strains The table below highlights bacteria strains that have been modified to enhance...Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified codons...

  28. AAV for Neuronal Tracing

    Type
    Collection
    ...deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Wickersham Introduction to ...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

  29. Viral Vectors

    Type
    Collection
    ...cancer cells. Adenoviruses have high transduction efficiency and direct high but transient transgene expression...clinical trials. These viruses have a relatively high transduction efficiency, though this infectivity... can be delivered to a host and generate virus (right), similar to how natural viruses can be delivered...stranded linear DNA >8 Kb 105 Dividing/Non-dividing No High γ-Retrovirus Stable RNA <8 Kb 80-130 Dividing Yes...

  30. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids ... and lentiviral vectors as in-stock preps. These high-titer preps are distributed as 100 µL solutions ...particles that are suitable for in vivo use. Order a high-quality and ready-to-ship AAV or lentivirus prep...services to you, we must ensure that you have the right to use the original plasmid. Distribution of the...

  31. Distribution to Industry

    Type
    Collection
    ...Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly.... biological materials and intellectual property rights, and provides liability protection for material...qualified for-profit entities. Addgene reserves the right to determine if an entity is qualified to receive...

  32. Worm Expression Resources

    Type
    Collection
    ...types and/or developmental timeframes. Here we highlight some of the plasmids and tools available at Addgene...Plasmid Tools for Use in Worms The table below highlights plasmids that contain tools for use in worm research...

  33. Viral Production

    Type
    Collection
    ...optimized for each specific virus in order to generate high quality preparations. After production, all virus...titers obtained using ddPCR are generally 2–3 fold higher than those achieved using the standard qPCR method...digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing and standardizing...staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins...

  34. Rett Syndrome

    Type
    Collection
    ...mutations are known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W... labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

  35. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ...

  36. AAV Viral Preps

    Type
    Collection
    ...the lowest biosafety protection levels. High quality and high titer in-stock AAV preps are affordable,... density centrifugation. Addgene’s comprehensive high-quality quality control process includes titering...

  37. Institute for Protein Innovation

    Type
    Collection
    ...combination allows IPI to generate antibodies to highly conserved proteins and other difficult-to-target...part of that mission, IPI is providing access to high-quality, well-characterized antibodies in an open...) Glypican Antibodies Glypicans are a family of highly conserved proteoglycans crucial for regulating ...

  38. Chemogenetics Plasmids

    Type
    Collection
    ...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

  39. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...

  40. Recombinases AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of... Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore...

  41. Antibody Production

    Type
    Collection
    ... for each specific antibody in order to generate high quality preparations. Following production, all ...transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...

  42. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Core) and Addgene previously partnered to provide high-quality AAV vectors to the academic research community...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...James M. Wilson AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen...

  43. TALEN Guide

    Type
    Collection
    ... are able to recognize their targets with fairly high specificity. Fifteen years of research has shown...TALe research has been swift. The incentives are high. Since ZF targets are confined to sequences composed...on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation of ...

  44. Neurodegeneration Research Collection

    Type
    Collection
    ...and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform...dissemination to the research community. This collection highlights plasmids created with support from the BRAIN ...

  45. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase...recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre...

  46. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... clones for loss of function. This is due to the high frequency of indels formed on non-deleted alleles...cells in order to enrich for cells that received high levels of the CRISPR/Cas9 constructs. Plate sorted...the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols...

  48. TREAT-AD Plasmid Collection

    Type
    Collection
    ...resources for emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized...further translational investment. All resources and high-quality research tools are shared openly. Plasmids...

  49. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... plasmid with or without expression of Cas9. Highlighted below are some multiplex gRNA vectors from our...Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and...

  50. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...a variety of popular plasmids and backbones with high cloning potential. By making these plasmids available...spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations. Can I directly transfect...
Showing: 1 - 50 of 986 results