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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 989 results for: IGH@;

Showing: 81 - 120 of 989 results

  1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...range between 0 and 100) are then converted to a weight (which ranges between 0 and 1) so that they can...ranging from -40 bp to 40 bp) are also converted to a weight (which ranges between 0 and 1) (shown in the graph...Conclusion CrispyCrunch is now publicly-available for high-throughput design and analysis of CRISPR experiments...

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...hand, there are reasons why grad school might not be the right choice. I spoke with our Financial Operations...here. You also don’t have to go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was.... I thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be up to date...biggest takeaways from my co-op. It has been so insightful to learn about different career paths, successes...

  3. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...oneself that prompted us to create Crowdfight.   Figure 1: Crowdfight is a community of scientists who...making science.   How we started Crowdfight-COVID19 Crowdfight was born as a collaborative platform...collaborator for a specific task, Crowdfight will: 1) Find an expert with the right skill; 2) Confirm the expert...Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020, while most of the world was locked...did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform the experiments... them heard about our online platform, called Crowdfight, and filed a request for help. In just a few ... a research group in Scotland, who had just the right infrastructure and offered to collaborate with them...

  4. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...researchers, we wondered how we could make the insights gained during this time more visible and thereby... ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video to learn ... ground again and again as they don’t save the insights they glean from a reading session. To remember...hunting through a whole folder of papers to find the right experiment! The ReFigure extension also tells readers...ReFigure making it easy for others to discover your insights on a topic. Beyond collating other’s data, you...include your own data in a ReFigure. For example, you might include a confirmatory image to show that a previously...previously used reagent performs well, or you might publish a replication experiment that you conducted ...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...the challenge of trying to figure out what system might work best for your particular purposes. We’re here...than others. In today’s post, we will focus on a high-level comparison of popular cloning methodologies...results. If you’re in a hurry, Golden Gate cloning might be for you. This methodology relies on versatile... in as little as 30 minutes! Alternatively, you might consider Gibson cloning. In this technique, you ...fragments in an order determined by their homology. You might also consider FastCloning, first described by Chaokun...approach should you use if the budget is particularly tight? Most cloning processes will require a PCR reaction... job done, provided that you’ve already got the right enzymes laying around the lab. If not, you’ll be...

  6. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...and wrap the column with blue LED lights. When you switch off the lights, OptoNBs bind YFP and non-specific... the blue lights. Which option do you choose?  Option #2 may sound like fiction, but light-controlled...dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table 1: Summary of the... effect of AsLOV2 insertion site on light-induced binding.  Light controlled protein purification with...while cycling between dark and blue light which revealed a blue light-dependent shift in mCherry fluorescent...cells: light-based control of signaling pathways. This is particularly exciting because using light to control...binding of their target protein is altered by blue light illumination. This ability to reversibly bind and...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...engineering of fusion proteins is relatively straightforward. However, generating a fusion while keeping...discuss strategies to generate fusion proteins and highlight some aspects of their design.  Protein size and...situation Next to size, potential homodimerization and higher order oligomerization of fluorescent proteins is... the fluorescent tag should operate as an inert light emitting module, any tendency of GFP to homodimerize...fluorescent protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity...termini of your protein of interest The most straightforward fusion protein is made by connecting the N-...the fluorescent protein-POI fusions, we generate straight fusions, without any linker. When both orientations...

  9. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ... focused on three different levels of assembly: High efficiency and accurate single-insert assembly Intermediate...destination plasmid. Antibiotic selection allowed for high throughput cloning efficiency measurements. We performed...confirmed the insertion of the Lambda insert at the same high frequencies as the antibiotic selection markers....lacZ cassette assembly was easily achievable with high levels of transformants and low backgrounds – so...Using these 4 bp overhang observations, accurate “high-fidelity” junction sets for both the 12- and 24-...complex assemblies were performed. It was found that high efficiencies and accurate assembly levels were indeed...in the number of transformants while maintaining high levels of correct assemblies) translate to faster...

  10. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ... (blue light-inducible AraC dimers in E. coli) that turns expression on and off with blue light.   Inspired...domain with VVD, a light-responsive domain that dimerizes upon exposure to blue light (Fig. 1). Another...VenturaIf you follow Barbara Di Ventura on Twitter, you might have seen the video of her singing about the paper...Another blue light-inducible dimerization domain, Vaucheria frigida aureochrome1 (VfAu1), led to functional...most applications.   Figure 1: With blue light, the VVD domains of the BLADE dimerize and activates...under the pBAD promoter, can be controlled with light without the need of any cloning.     What advantages...BLADE activates transcription with the flick of a light switch and shuts off just as quickly. The original...

  11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...This is where FLEx enters the scene. FLEx: A light when night is about you! The FLEx (for flip-excision)...optogenetics? Optogenetics: Controlling neurons with light In 2005, a team from Stanford University, led by...ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb photons for energy...maintain membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins...2000). Most neurons in the brain are not naturally light-sensitive, so the selective expression of opsin ...Optogenetic neuromodulation is made possible by the light: when hit with the correct wavelength, microbial..., 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins that, in turn, trigger...

  13. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...Scientist. I used to spend my days (and often my nights :D) engineering E. coli to produce biofuels in ... screaming from the lab like the cartoon on the right?!? I would love to tell such a dramatic story, but...This may come as somewhat of surprise to many of my high school friends. In my teenage years I was super ...can involve walking alongside it and giving it a high five. While screaming can be therapeutic and I’ve...

  15. NETRF

    Type
    Collection
    ... NETRF believes that creating and maintaining a highly collaborative NET research community is pivotal...

  16. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain...

  18. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...resulting in frameshifts. If a repair template with high homology to the DNA surrounding the DSB is introduced...

  19. Fluorescent Protein Guide

    Type
    Collection
    ...fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals...

  21. All Antibodies

    Type
    Collection
    ...for Protein Innovation Collection : Antibodies to highly conserved proteins and other difficult-to-target...

  22. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... target various genes or genomic regions. It is highly recommended that you determine if your cell type...

  24. TALEN Engineering

    Type
    Collection
    ...FLASH (Fast Ligation-based Automatable Solid-phase High-throughput) assembly method (Reyon and Tsai et al...

  25. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been ...

  27. Microbiology Resources

    Type
    Collection
    ... in bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome...

  29. p53 Pathway

    Type
    Collection
    ... The p53 name arose from its observed molecular weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact...

  30. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain...

  31. Arf GTPase Family

    Type
    Collection
    ... Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L....

  32. Control AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...

  33. Ras Pathway

    Type
    Collection
    ...erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 ...

  34. Lentivirus Plasmids

    Type
    Collection
    ...transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd EF-1alpha driven...

  35. CRISPR Plasmids - Tagging

    Type
    Collection
    ...vector serves as a backbone to clone the left and right homology arms. More details on how to use this plasmid...

  38. Retrograde AAV viral preps

    Type
    Collection
    ...Looger 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate sensor Glutamate sensor Looger...

  39. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Protein Activation Library 113345 Activation Human Wright 3rd 7-14 58,570 Human miRNA CRISPR Knockout Library...

  40. Plasmids for Stem Cell Research

    Type
    Collection
    ... Lin28A to somatic cells from separate plasmids Highly Efficient Reprogramming to Pluripotency and Directed...
Showing: 81 - 120 of 989 results