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We narrowed to 964 results for: Abo

Showing: 101 - 150 of 964 results

  1. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins. Optional...concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr... of the tip of the pipette into the buffer just above the well. Very slowly and steadily, push the sample... of the way down the gel. A typical run time is about 1-1.5 hours, depending on the gel concentration ...

  2. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing... not already been done. We typically start with about 250 mL of supernatant and add 250 µL of 100X protease... sample among multiple columns. For information about the total binding capacity for your columns, refer...setting. If the concentration of the pooled sample is above 1.0 mg/mL proceed to Option 1 with a buffer exchange...based on the concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column...

  3. Video Library

    Type
    Protocol
    ... Protocols Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization... MTA FAQs Answers to frequently asked questions about the MTA process Help Center How to Deposit Your ...projects in the works, and all of the things she loves about working at Addgene! Maria Soriano Maria Soriano,...in science, the importance of international collaboration, and offers career advice for scientists looking...

  4. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the Video...maintenance and use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, ...the pipette Watch the Video! Centrifugation Learn about selecting and using a centrifuge to separate different... a liquid sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing...Weighing Reagents Learn how to weigh laboratory materials on a balance Basic Molecular Biology Name Description...

  5. Centrifugation

    Type
    Protocol
    ...Learn about selecting and using a centrifuge to separate different components in a liquid sample....Introduction A centrifuge is a common piece of laboratory equipment used to separate different components.... Floor Centrifuges sit on the floor and can be about bench height. They often spin at similar speeds ...tabletop microcentrifuge like the one in the picture above. Wear PPE appropriate for the lab space in which...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ....8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste container...in a total of 5 mL of cell lysis buffer (recipe above). Pipet back and forth to resuspend each pellet ...pellets and keep on ice. Process the cell pellet from above as follows: Resuspend and lyse the cells by adding...tube containing the resuspended virus from step 12 above. Add 50 units of benzonase per mL of viral suspension...

  7. Weighing Reagents Protocol

    Type
    Protocol
    ...balance in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. ... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...

  8. Immunocytochemistry

    Type
    Protocol
    ...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may...produce the same phenotype will alleviate concerns about off-target effects. B.2 Ordering Oligos Compatible...should be decontaminated with at least 10% bleach. Laboratory materials that come in contact with viral particles...work in a BL2 facility. If you have any questions about what safety practice to follow, please contact your...

  10. Protocol - How to Design Primers

    Type
    Protocol
    ...amplification process. When designing, if unsure about what nucleotide to put at a certain position within...capabilities. Taking into consideration the information above, primers should generally have the following properties...

  11. Protocol - How to Streak a Plate

    Type
    Protocol
    ...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...a section of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...coli after Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin...concentrations that will work for this assay, and the above result represents a single experiment. For publishable...

  13. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link....sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email help@...

  14. Water Bath Protocol

    Type
    Protocol
    ... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...even if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate...

  15. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...verification, such as DNA sequencing . In the example above, digestion with enzyme RE1 will linearize the 6200bp...that you get double confirmation. In the example above, digestion with either RE3 or RE4 will give a very...

  16. Using a Light Microscope Protocol

    Type
    Protocol
    ...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

  18. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...approximately 50–60% confluent. For 293T cells this is about 2 × 10 6 cells in a 10 cm dish. Each 10 cm dish ...cell solution into the conditioned medium prepared above to make a new cell solution at a concentration of...

  19. Kit Free RNA Extraction

    Type
    Protocol
    ... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...

  26. Gibson Assembly Protocol

    Type
    Protocol
    ... the right). When designing your plasmid, think about what DNA segments you will need to join to create...

  32. AAV ddPCR Titration

    Type
    Protocol
    ...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge...

  33. CRISPR Library Amplification

    Type
    Protocol
    ...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...

  34. Addgene's eBook Collection

    Type
    Guide
    ... | Fourth Edition About Plasmids 101 September 2023 | Fourth Edition About Based on our popular...techniques! Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January...screens. Close About Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent... more! Close About Overlay Select Viral Vectors 101 July 2024 | Second Edition About Viral Vectors...more! Close About Overlay Select Science Career Guide November 2018 | First Edition About Science Career...Close About Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR... trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay ...

  35. Antibody Guide

    Type
    Guide
    ...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...basic protocols and controls follow that of IF (above). Sample preparation, signaling excitation, and ...

  36. Chemogenetics Guide

    Type
    Guide
    .... However, CNO is back-metabolized to clozapine and other clozapine metabolites and these can have off-target...concentrations of clozapine N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics Guide . ...tissue-specific Cre is provided. Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors...

  37. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors...of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...increased safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus...packaging methods . The three plasmids described above (envelope, packaging, and transfer) are co-transfected... Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S., Su, L.,...

  38. Molecular Biology Reference

    Type
    Guide
    ...human genome with restriction enzymes would yield about two million DNA fragments, which is far too many...the molecular biologist’s toolbox. To learn more about different types of cloning methods check out our...restriction enzyme digestion. For more information about restriction enzymes check out NEB's website . Insert...endogenous expression levels). For more information about promoters, both bacterial and eukaryotic, as well...can be replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term...codon. Below you will find helpful resource tables about the genetic code. This table includes the nucleotide...

  39. Lentiviral Vector Guide

    Type
    Guide
    ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors...gamma-retroviral packaging systems, and vice versa. For more about gamma-retroviruses, see Addgene's gamma-retroviral...are necessary for lentiviral production in the laboratory, and many have been removed or mutated for increased... genome. To produce lentiviral vectors in the laboratory, the non-required components have been removed...

  40. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...our adeno-associated viral vector guide to learn about AAV vector components, production and common uses...E2a and VA for replication. For more information about adenovirus, read our Adenoviral Vector Guide . Recombinant... factories for the new viral particles in the laboratory. The cell line used for rAAV vector production...necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog post on ...in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics 101 video...

  41. Educational Resources

    Type
    Guide
    ...biology, laboratory research, and career development. eBooks Download any of our eBooks to learn about popular...Playlists include: Protocols Careers How-to Blog Read about plasmids in Addgene’s repository , and related molecular...Technology Career Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes...

  42. CRISPR Guide

    Type
    Guide
    ...and are discussed in more detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA...Nme2Cas9 (from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than...targeting range for plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity...convert one base to another within a target locus of about 8 bp, specified by a gRNA. There are two main types...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...

  43. Plan Your Experiment

    Type
    Guide
    ...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable...when carrying out CRISPR experiments. Read more about how to design your gRNA . Browse Plasmids: gRNAs...to optimize your delivery conditions. Learn more about CRISPR delivery in mammalian systems . Validate ...

  44. Adenovirus Guide

    Type
    Guide
    ...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...E2a and VA for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral...linearized construct, and rAdV vectors can be harvested about 7–10 days later. Vogelstein designed multiple shuttle...

  45. Science Guides

    Type
    Guide
    ...the following guides to help you start learning about various scientific concepts related to our plasmid...Viral Vectors Find educational guides to learn more about the different types of viruses that are commonly...

  46. Guide to Using Pooled Libraries

    Type
    Guide
    ... to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used. Below...simultaneously in a single reaction. As discussed above, access to this technology is crucial for conducting...

  47. Promoters

    Type
    Guide
    ...of interest is expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

  48. Optogenetics Guide

    Type
    Guide
    ... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

  49. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...keep spreading the word about materials sharing, but we don’t have to worry about getting support from depositors...of course always being vigilant about resources, I’m not worried about the stewardship of this in the ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...on this. Addgene’s success is about the Addgenies. I never worry about their dedication, excellent intentions...more about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our ...a lot of things we actually don’t have to worry about and that is something to recognize! Distributing...the last couple of years, we don’t have to worry about having enough space! There’s enough space here at...

  50. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...interesting content Talk about something you know a lot about and that you care about. Don’t try to present...engaging and memorable. If you read my story above about the presentation faux pas, you are more likely...the room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs ...dynamite presentation?  To that end, let’s chat about giving science talks and how to make them serve ...other people give great talks We are not talking about lab meeting here. Lifeless presentation of experimental...You want to give the audience something to think about, argue with, or learn from. Some of the best talks...topics many times, but I always seek out information about the particular audience and venue before tailoring...
Showing: 101 - 150 of 964 results