We narrowed to 990 results for: Abo

...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...

...and Optogenetics Guide page for more information about this field. Use the table and filters below to find...

... vivo imaging or creating biosensors. Read more about BRET on our blog . Nano-Lanterns: Fluorescent proteins...

...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...

...BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed in addition... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves... materials. Video Watch the video to learn more about safety in the lab. Equipment Lab coat Close-toed... eat, drink, chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure ...Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some times...not be worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names...names and contact information of the laboratory manager should be clearly visible in the room and on the ...

...colors as needed for your experiments. Learn more about the flexibility of self-labeling tags on our blog...

...Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle...liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy...100 from top to bottom (as shown in the picture above), while 650 µL would read as 065. P200: The first...100 from top to bottom (as shown in the picture above), while 95 µL would read as 095. P20: The first ...10 µL would read as 100 (as shown in the picture above), while 2.2 µL would read as 022. P2: The first ...1 µL would read as 100 (as shown in the picture above), while 0.5 µL would read as 050. Each volume display...

...cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins. Optional...concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr... of the tip of the pipette into the buffer just above the well. Very slowly and steadily, push the sample... of the way down the gel. A typical run time is about 1-1.5 hours, depending on the gel concentration ...

...personal protective equipment including laboratory coat, laboratory goggles, and gloves. Sodium azide containing... not already been done. We typically start with about 250 mL of supernatant and add 250 µL of 100X protease... sample among multiple columns. For information about the total binding capacity for your columns, refer...setting. If the concentration of the pooled sample is above 1.0 mg/mL proceed to Option 1 with a buffer exchange...based on the concentration of the pooled sample above. Option 1: Buffer exchange using a desalting column...

... Protocols Video protocol guides for standard laboratory procedures. (Link opens in a new window) Video...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization... MTA FAQs Answers to frequently asked questions about the MTA process Help Center How to Deposit Your ...projects in the works, and all of the things she loves about working at Addgene! Maria Soriano Maria Soriano,...in science, the importance of international collaboration, and offers career advice for scientists looking...

...and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the Video...maintenance and use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, ...the pipette Watch the Video! Centrifugation Learn about selecting and using a centrifuge to separate different... a liquid sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing...Weighing Reagents Learn how to weigh laboratory materials on a balance Basic Molecular Biology These protocols...

...Link opens in a new window) together with the laboratory of NeuroMab Director, Dr. James Trimmer, have...nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain...

...Learn about selecting and using a centrifuge to separate different components in a liquid sample....Introduction A centrifuge is a common piece of laboratory equipment used to separate different components.... Floor Centrifuges sit on the floor and can be about bench height. They often spin at similar speeds ...tabletop microcentrifuge like the one in the picture above. Wear PPE appropriate for the lab space in which...

....8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste container...in a total of 5 mL of cell lysis buffer (recipe above). Pipet back and forth to resuspend each pellet ...pellets and keep on ice. Process the cell pellet from above as follows: Resuspend and lyse the cells by adding...tube containing the resuspended virus from step 12 above. Add 50 units of benzonase per mL of viral suspension...

...balance in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. ... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...

...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade...

...Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited pLKO derived vectors that may...produce the same phenotype will alleviate concerns about off-target effects. B.2 Ordering Oligos Compatible...should be decontaminated with at least 10% bleach. Laboratory materials that come in contact with viral particles...work in a BL2 facility. If you have any questions about what safety practice to follow, please contact your...

...amplification process. When designing, if unsure about what nucleotide to put at a certain position within...capabilities. Taking into consideration the information above, primers should generally have the following properties...

...resistance and your initials. Labeling within a laboratory setting is important for organization, and it...a section of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick...

...coli after Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin...concentrations that will work for this assay, and the above result represents a single experiment. For publishable...

...Addgene lists the primers used to obtain each result above the posted sequence in the "View Sequence" link....sequence than expected and wish to contact Addgene about the accuracy of your plasmid, please email help@...

... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to ...even if you are using disinfectants as described above in step 3. You will also need to maintain the appropriate...

...verification, such as DNA sequencing . In the example above, digestion with enzyme RE1 will linearize the 6200bp...that you get double confirmation. In the example above, digestion with either RE3 or RE4 will give a very...

...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...window) New England Biolabs for more information about restriction enzyme buffers). If you select enzymes...

...approximately 50–60% confluent. For 293T cells this is about 2 × 10 6 cells in a 10 cm dish. Each 10 cm dish ...cell solution into the conditioned medium prepared above to make a new cell solution at a concentration of...

... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...

...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...

...and bathroom. These items should remain in the laboratory. When traveling between lab spaces, take your...

...the now gel purified DNA. Find more information about DNA quantification here . Tips and FAQ How do you...

...water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos with cut vector...

...suggested amount, instead of the other dry ingredients above. Loosely close the cap on the bottle (do NOT close...

... the right). When designing your plasmid, think about what DNA segments you will need to join to create...

...more at (Link opens in a new window) NEB's website about star activity . If you are digesting a large number...

... supernatant out of the tube if you are worried about losing the pellet. Dry with vacuum or by inverting...

...manufacturer’s instructions for specific instructions about extension time and temperatures. Initial Denaturation...

...cells in the untransduced well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor...

...Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same ...

...point. If your unknown sample’s absorbance falls above the range of the standard curve you will need to...

...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge...

...require modifications dictated by the originating laboratory for optimal results. If you obtained the pooled...

...keep spreading the word about materials sharing, but we don’t have to worry about getting support from depositors...of course always being vigilant about resources, I’m not worried about the stewardship of this in the ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...on this. Addgene’s success is about the Addgenies. I never worry about their dedication, excellent intentions...more about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our ...a lot of things we actually don’t have to worry about and that is something to recognize! Distributing...the last couple of years, we don’t have to worry about having enough space! There’s enough space here at...

...interesting content Talk about something you know a lot about and that you care about. Don’t try to present...engaging and memorable. If you read my story above about the presentation faux pas, you are more likely...the room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs ...dynamite presentation?  To that end, let’s chat about giving science talks and how to make them serve ...other people give great talks We are not talking about lab meeting here. Lifeless presentation of experimental...You want to give the audience something to think about, argue with, or learn from. Some of the best talks...topics many times, but I always seek out information about the particular audience and venue before tailoring...

...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...professors will not only talk about their own interests, but will ask you questions about your research goals and...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...this:   However, as simply as it seems laid out above, there are many places where the PhD application...-hand, you can quickly reference your questions about the PI’s research or the name of the award you won...new way to allow you to get important information about the campus even if you can’t visit. By talking with...department, the lab setting, and how students truly feel about the program and faculty. Here are some questions...

... because we are highly collaborative, we love teamwork. Working collaboratively allows our team to best...clarification and again we collaborate with the Product Team who in turn collaborates with stakeholders to ...Scrum framework is based on an iterative and collaborative approach to development. Scrum teams plan and...that it provides transparency to everyone involved about the status of each project. This creates accountability... amongst the team. Whether we are a highly collaborative team because we are using the Scrum framework...These initial stories are usually a result of collaborations between Addgene’s Product Team and the stakeholders...Once we begin work on a story, we continue to collaborate closely with each other and with other teams....

...dig deep Talk to your professors about their research, and learn about what makes them excited to do what...sciences), people conduct research to answer questions about how the world works. As scientists, we probe beyond...curious person. I like to ask questions and think about the best way to answer those questions. I’ve met...enjoy, is worthwhile and can help us make decisions about what we want to do in the future. How do I get started...sense of what kind of research you would be excited about. I know quite a few friends who opted to join labs... a lot out there worth exploring! Being excited about the science allows you to ask more interesting questions...as graduate students (your teaching assistants), about their experiences. Research requires dedication ...

...matters Yes, yes it’s all about the science but really it’s not. It’s about the atmosphere that will allow...and are nervous about discussing data. There’s hesitation before answering questions about lab culture. ... emotions and assumptions. I’m not just talking about simple things like “Which route should I take to... and not very communal. That didn’t suit my collaborative learning style at all. Finally I rotated in ... did). Some of the information you will need is about you. It’s worth taking time to honestly evaluate...lab members can give open, revealing information about the culture and lab head. While it’s good to talk...same department as well. What do they have to say about this lab’s reputation? If you are choosing a training...

...learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about the...ot­her disciplines, are even less likely to read about our work. We therefore must discover new ways to.... Only on occasion do we then have time to read about research from other disciplines, or from other countries...communication through video     Video abstracts about research, especially published research, have grown...researchers have Facebook pages, few share any details about their research itself. Pinterest is also a favorite...created a new platform for sharing video abstracts about research: www.WeShareScience.org. WeShareScience...platform for sharing short, 3 to 5 minute videos about research. Videos can be shared by research scientists...

...possessed. Think about your shining moments. Recall a few times you really felt great about your work. Maybe...having a life outside my job. The same can be said about teamwork, communication, writing, management, and...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...add value, not just “do the job”. When they ask about your creativity, your potential manager wants to...outside the box to solve a problem. When they ask about your communication style, your potential teammates...they have developed as part of a successful collaboration and fail to identify it as a personal strength...yourself in situations where you can learn more about possible strengths and weaknesses, and view everything...

...scientist anymore. Being a scientist is not about what you do--it’s about how you think. I have not worked at ...10 years, but when people ask me about my job, I have no qualms about saying “I’m a scientist.”    What...installment of the Addgene career guide, we’ll think about what causes this culture shock and how to overcome...counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues, we’ve...This is probably because you don't have to worry about experiments taking longer than expected or dealing... hour days at the lab, I encourage you to think about how much of that time you’re actually spending doing...candidates over the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is ...