We narrowed to 1,033 results for: CAT;

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

...implementation letter DNA amplification Viral vector production Density centrifugation purification Addgene’s comprehensive...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...order ships. Shipping time will depend on your location. Share Your Results Once you start getting results...high-quality viral vector preps at Addgene, including purification, titration, and quality control measures. We...

... Return to Top Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... Tet-On 3G transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing...-tag from TRE3G promoter for Tandem-Affinity Purification. Yannick Doyon 20321 TetO-FUW-OSKM Tet-On inducible...

...a ncAA into a protein of interest, four key modifications to the standard translation machinery are required...with a different amino acid. To achieve these modifications, two plasmids are generally used. Once these...amino acids (ncAA) in a protein of interest. Applications By making small changes in selected amino acids...code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3) ΔserC serC...gene replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS...genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS...Davis L. & Chin J. W. (2012). Designer proteins: applications of genetic code expansion in cell biology . ...

...contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid...multi-purpose toolkit that enables targeted, specific modification of monocot and dicot genomes using TALENs and...Successfully validated in tomato, tobacco, Medicago truncatula , wheat, and barley. Return to top Gene Silencing...organelles, membranes and cytoskeletal elements in M. truncatula . Return to top Other Plant Resources Addgene...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...Golden Gate TALEN kit. The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed...in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and C-terminus of the...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...expression in mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller...

... disruptions, missense mutations located in the MBD, and truncations prior to the NID are more severe ...even within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...

...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...defined by their location, their pathway dependencies are extremely important for classification and treatment...network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential ... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, read...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

...the functional catagories listed below. Narrow down the items available within a category by using the ...experiments can be found on the material pages for viral catalog items. Browse Available Retrograde AAV See our ... Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

...cited in publications. All pricing includes DNA amplification, viral vector production, purification, and ...Look up the IDs of particular Penn Vector Core catalog items. Virus-associated DNA Each viral vector service...

...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...used to transduce cells of interest. Specific modifications to these plasmids, such as different promoters...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

...Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...the Allen Institute for Cell Science Cell Catalog (Link opens in a new window) . Protocols The Allen Institute...: 28814507 (Link opens in a new window) More publications from the Allen Institute for Cell Science (Link...

...purpose-driven nonprofit, Addgene offers expert support, educational resources, and efficient material transfer solutions...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...developed for special functions that vary from purification, visualization, and functional studies. You ...

...copGFP from a truncated EF1 promoter pCDH-EF1S-Nluc 73033 Expresses Nluc from a truncated EF1 promoter ...pCDH-EF1s 72484 Express gene of interest from a truncated EF-1 promoter pCDH-EF1-Luc2-P2A-copGFP 72485 Expresses...pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated EF1 promoter pCDH-CMV-Nluc-P2A-copGFp-T2A-Puro...

... Protein PI 71367 Human Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens junctions beta-Catenin mCherry* Michael Davidson 30313...Fluorescent Protein Collection or search our full catalog for a marker for your structure of interest. Additional...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...programming sgRNA and HR donors for selectable genome modification. This system can be used to create C- and N-...following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection...

...Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs.... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication...

...to find antibodies currently available from our catalog that target epitope tags and other common cellular...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

...synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing and Signaling... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

...to find antibodies currently available from our catalog that target proteins popular in neuroscience research...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

... variation. These joint efforts will produce a catalog of the impact of genomic variants on genome function...Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF Consortium (Link...opens in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...

... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...experiment. Browse Fluorescent Protein Plasmids by Category Empty Backbones Study your protein of interest...

...Visualize Genomic Loci Mammalian Resources Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...

...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...

...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...

... by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including... , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and...

...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. ...

...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended...

...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...posted on the material page for the corresponding catalog item (see maps section for images). Figure 2: AAV...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available...

... plasmids to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression...monitor expression. Luk Parijs 11619 pLB 3rd Modification of pLL3.7 with added genetic elements known ...3rd For barcoding transcription factors for identification in pooled screens. Part of the MORF Collection...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Description Gene/Insert Antibiotic Resistance PI Publication Back to Top Do you have suggestions for other...

...Xenopus". Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...

... Plasmid Gene/Insert Vector Type Promoter PI Publication Return to top CRISPR Resources Addgene has a ...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page...