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We narrowed to 1,030 results for: CAT;

Showing: 101 - 150 of 1030 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression...expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

  2. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

  3. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

  4. Antibodies

    Type
    Collection
    ...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...Additionally, we have developed a variety of educational resources to help you choose and use antibodies...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

  5. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...binding. Editors carrying the delta-984-1090 ADAR2 truncation retain RNA editing capabilities but are small...systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that...

  6. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR...CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing...plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ... of applications. To learn more and plan your experiment, check the plasmid's original publication or ...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  8. AAV Molecular Tools

    Type
    Collection
    ... Tools for Affinity Purification These AAV encode tools for affinity purification (which can be used for...tet-off transactivators and tools for affinity purification (TRAP). Viral... Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty Prime...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  10. Microbiology Resources

    Type
    Collection
    ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  11. AAV Packaged on Request

    Type
    Collection
    ...implementation letter DNA amplification Viral vector production Density centrifugation purification Addgene’s comprehensive...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...order ships. Shipping time will depend on your location. Share Your Results Once you start getting results...high-quality viral vector preps at Addgene, including purification, titration, and quality control measures. We...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... Tet-On 3G transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing...-tag from TRE3G promoter for Tandem-Affinity Purification. Yannick Doyon 20321 TetO-FUW-OSKM Tet-On inducible...

  13. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate TALEN kit. The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed...in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and C-terminus of the...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...expression in mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller...

  14. Genetic Code Expansion

    Type
    Collection
    ...generally introduced in the media. A new codon to be allocated to the new amino acid. Because there are no free...single plasmid, with multiple copies of the tRNA. Applications By making small changes in selected amino acids...tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls must be used to...code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3) ΔserC serC...gene replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS...genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses archaeal MjTyrRS...

  15. Rett Syndrome

    Type
    Collection
    ... disruptions, missense mutations located in the MBD, and truncations prior to the NID are more severe ...even within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...the functional catagories listed below. Narrow down the items available within a category by using the ...experiments can be found on the material pages for viral catalog items. Browse Available Retrograde AAV See our ... Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  17. Cancer Research Plasmids and Resources

    Type
    Collection
    ...defined by their location, their pathway dependencies are extremely important for classification and treatment...network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential ... described in the initial Target Accelerator publications, focused on somatic variants found in lung adenocarcinoma...

  18. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Gene/Insert PI Publication Do you have suggestions for other plasmids that should be added to this list?...

  19. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSBs) at a specific location based on a co-expressed gRNA-defined target sequence... changes. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...but their function is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids...

  20. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins (FPs) and to find the...scientists. Browse Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and...Database hosted at the University of Arizona Zeiss Education in Microscopy and Digital Imaging Olympus Fluorescent...

  21. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ... Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...but their function is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids...

  22. Institute for Protein Innovation

    Type
    Collection
    ...Antibody Description Isotype Reactivity Recommended Applications Integrin Antibodies Integrins are a family of...Antibody Description Isotype Reactivity Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope tags and other...Antibody Description Isotype Reactivity Recommended Applications Browse Antibody Plasmids The following plasmids...

  23. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January 30, 2025 Do you have suggestions for other...

  24. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...

  25. AAV for Neuronal Tracing

    Type
    Collection
    ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  26. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type Each...used to transduce cells of interest. Specific modifications to these plasmids, such as different promoters...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

  27. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...the Allen Institute for Cell Science Cell Catalog (Link opens in a new window) . Protocols The Allen Institute... PubMed All Allen Institute for Cell Science Publications (Link opens in a new window)...

  28. Promega Plasmid Collection

    Type
    Collection
    ...purpose-driven nonprofit, Addgene offers expert support, educational resources, and efficient material transfer solutions...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...developed for special functions that vary from purification, visualization, and functional studies. You ...

  29. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Protein PI 71367 Human Beta-catenin GFP Adherens Junctions Beta-catenin EGFP Alpha Yap 67937 mouse E-...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens Junctions Beta-Catenin mCherry Michael Davidson 30313...chaperones to help with folding or post-translational modification to function properly. Browse or search the table...

  30. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...copGFP from a truncated EF1 promoter pCDH-EF1S-Nluc 73033 Expresses Nluc from a truncated EF1 promoter ...pCDH-EF1s 72484 Express gene of interest from a truncated EF-1 promoter pCDH-EF1-Luc2-P2A-copGFP 72485 Expresses...pCDH-EF1s-copGFP 73034 Expressed copGFP from a truncated EF1 promoter pCDH-CMV-Nluc-P2A-copGFp-T2A-Puro...

  31. CRISPR Plasmids - Tagging

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...programming sgRNA and HR donors for selectable genome modification. This system can be used to create C- and N-...following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection...

  32. COVID-19 Resources

    Type
    Collection
    ... CD147-spike protein . Cathepsin L - Bosch BJ, et al, 2008, J Virol. Cathepsin L Functionally Cleaves ... is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine endosomal protease that triggers...Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-CoV-2, including with ...

  33. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...important variables include: Type of genetic modification - CRISPR libraries vary based on whether they...includes information about the type of genetic modification and target species. Additional information about...Human Sabatini and Lander 3rd 10 187,535 Advanced Catalogue of Epigenetic Regulators (ACER) 226117 Knockout...

  34. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs.... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication...

  35. Tags and Other Markers

    Type
    Collection
    ...to find antibodies currently available from our catalog that target epitope tags and other common cellular...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

  36. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing and Signaling... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

  37. Neuroscience

    Type
    Collection
    ...to find antibodies currently available from our catalog that target proteins popular in neuroscience research...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

  38. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... variation. These joint efforts will produce a catalog of the impact of genomic variants on genome function...Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF Consortium (Link...opens in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...

  39. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...

  40. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

  41. CRISPR Plasmids - Parasites

    Type
    Collection
    ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ... ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library...

  42. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...

  43. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

  44. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...

  45. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...

  46. Synthetic Biology - Overview

    Type
    Collection
    ... by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including... , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and...

  47. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. ...

  48. Plant Plasmids and Resources

    Type
    Collection
    ...Multipurpose Toolkit enables targeted, specific modification of monocot and dicot genomes using genome engineering...engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel Voytas. A CRISPR...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

  49. Open Enzyme Collection

    Type
    Collection
    ...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended...

  50. Viral Production

    Type
    Collection
    ...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...posted on the material page for the corresponding catalog item (see maps section for images). Figure 2: AAV...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available...
Showing: 101 - 150 of 1030 results