We narrowed to 210 results for: cycs

1. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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   Blog Post

   ...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...

2. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ...is present, the probes will ligate. Subsequent cycling through rounds of melting and annealing allows ...

3. 9 tips for a successful postdoctoral experience

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   Blog Post

   ...presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation...

4. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   ... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor...

5. Typing CRISPR Systems

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   Blog Post

   ... depth, the Jennifer Doudna lab maintains an encyclopedia of Class 2 CRISPR systems called CasPEDIA, with...

6. Antibodies 101: Flow Cytometry

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   Blog Post

   ...modifications, determining cell health, analyzing cell cycle stages, and detecting specific populations of cells...

7. A Needle in a Base-Stack: Cas9 Structural Biology

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   Blog Post

   ...many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et...

8. CRISPR 101: Multiplex Expression of gRNAs

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   Blog Post

   ... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...

9. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   Blog Post

   ...harmful mutations More, due to selection from more cycles of proliferation Less Cell types All types Less...

10. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...reversible and repeatable when blue light (450nm) was cycled on-off-on. Using a slit photomask, the researchers...

11. Delivery Methods for Generating iPSCs

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    ...replicates in the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s ...

12. Cre-lox system

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    ...Iwasato 85577 pTC-CMV-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT...Ehmer 85578 pTC-ApoE-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression ApoE.HCR.hAAT...

13. Using AAV for Neuronal Tracing

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    Blog Post

    ...cells and to the uncoating step in the viral life cycle. Using AAV for neuronal tracing AAV vectors are ...

14. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...stripping, since you’ll lose protein with every strip cycle. Troubleshooting the troubleshooting There’s many...

15. CRISPR Plasmids - Epigenetics

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

16. CRISPR Plasmids - Repress Gene Expression

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

17. CRISPR Plasmids - RNA Editing

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing...

18. CRISPR Plasmids - Activate Gene Expression

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

19. CRISPR Plasmids - Single-Strand Break (Nick)

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

20. CRISPR Plasmids - RNA Targeting

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing...

21. AAV Viral Preps

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    ...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...

22. CRISPR Plasmids - Double-Strand Break (Cut)

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

23. CRISPR Plasmids - gRNAs

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

24. CRISPR Plasmids - Base Edit

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    ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

25. Retrovirus Plasmids

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    ...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

26. mTOR Pathway

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    ...translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation...

27. Microbiology Resources

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    ...new window) National Collection of Yeast Cultures (NCYC) (Link opens in a new window) NIH AIDS Reagent Program...

28. Worm Expression Resources

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    ... genetic model organism due to their rapid life cycle (3 days from egg to adult) and easy lab maintenance...

29. CRISPR Guide

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    ...efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

30. CRISPR References and Information

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    ...Resources CasPEDIA (Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries ...