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We narrowed to 754 results for: REV

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Showing: 201 - 240 of 754 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to...the HIV-1 genome from every infected cell and 2. prevent reinfection of those cells. Kaminski et al. designed...HIV-1 cleavage allows T-cells to survive, but preventing re-infection would require sustained expression...infects over 250 million people worldwide. As we’ve previously seen in the CRISPR field, the lessons learned...

  2. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...environment. We’ve covered five model organisms in a previous blog post, but here are five more model organisms...conserved across species, studying the axolotl may reveal the potential humans have for a similar degree ... the sequencing of the entire axolotl genome, revealing an abundance of genes related to limb regeneration...Model for Regeneration and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... But conflict can also be good. In science, a reviewer’s critical comments on your manuscript is a form.... As you perform the experiments to address a reviewer’s concerns, you are making your hypothesis stronger...majority of conflicts I address in my current role revolve around missed tasks, stalled projects, and disagreements...particular teams at that given time. I think about how previous iterations of those teams resolved similar issues...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...their publications or as a review on a company website. It is important to review the data and protocols ...Uhlen, et al. 2016 is a good place to start.) Reviewing available antibodies can seem overwhelming, especially...available resources and give yourself time to carefully review your options. If possible, try to select a couple...

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...have higher apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1)....protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For detection of...have lower background and higher contrast than previously developed NIR split probes (Tchekanda et al.,...near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021).   Table 1: Properties...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get...primer and the downstream restriction site to the reverse primer. Next, we need to examine the DNA sequence...homology to the ORF). We now need to generate the reverse-complement of this sequence so that we can successfully...successfully amplify the ORF. You can generate the reverse-complement using existing software (a quick internet...others). If we put the sequence we chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA...digestion, you will need to use a phosphatase to prevent re-circularization of the vector. You should treat...

  7. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...protein. Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More...Protein (GFP) and other fluorescent proteins revolutionized live-cell protein detection, their bulk (~27...which lead to cystic fibrosis, one of the most prevalent inherited diseases. The authors investigated the...

  8. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...rAAVs, can sound almost too good to be true! In a previous post, we covered systemic capsids, which allow...1, rAAV2/2, and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish...(also known as AAVrg) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is ...variables, so be sure to check the literature for previous work in your experimental system. Table 2: AAV...

  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...sugar-containing molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology... have visible colonies growing on the membrane wherever an E. coli colony expressing a functional binding...  References 1. Nobrega, Franklin L., et al. "Revisiting phage therapy: new applications for old resources...Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David...

  10. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive...the LOV domain blocks the enzyme binding domain preventing downstream signaling pathways. Light exposure...optimize the intensities of light sources used to prevent activation of both tools with each light source....  Finding the right information When using a previously published system in your own work, you’ll need...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...silent, they could identify the target genes of previously uncharacterized enhancers. The popular TALEN-...CRISPR activators and repressors are thought to be reversible once the effector is inactivated/removed from...KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced methylation...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...or not. Choose wisely This point ties into the previous one too. I would recommend applying for multiple...factors that can make or break a lab experience. Previous Addgene blogs have discussed the process that ... but I’ve learned the value in finding mentors wherever I go. This story by Dr. Erika Moore in Science...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...suitable for targeting FRET acceptors to proteins (reviewed in [Yan & Bruchez, 2015]). To get you started,...HTRF: a technology tailored for drug discovery–a review of theoretical aspects and recent applications....energy transfer as a spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID...

  14. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...yeast, flies, and even plants! In this blog we will review common uses of MoClo and the kits Addgene offers...the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015). This kit...2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression, ...Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American...

  15. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  16. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  17. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  20. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...of plasmid prep do you need? As we covered in a previous post on plasmid preps, not all plasmid preps are...purification kits make it easier than others to prevent quality issues. For example, some kits reduce the...weight of the package during shipping. Of the kits reviewed here, NEB’s Monarch®, QIAGEN’s QIAwave, and Milllipore... high level of consistency between lots, then reviewing their quality assurance documentation can help...

  22. CRISPR Plasmids - Plants

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  23. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  25. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  26. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...case letter to the beginning of their insert abbreviation to specify what species it is. Example: ‘h’ ...amino acid mutation is to list the one letter abbreviation of the wild type amino acid immediately followed... Methionine (Met) followed by the one letter abbreviation of the mutated amino acid currently at that ...

  28. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  29. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  31. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  35. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...Annals of Internal Medicine published systematic reviews on an agent used to promote bone growth based on... open access as it does about the state of peer review. 8)     That same issue of Science also included...showing overwhelming support for open access to peer-reviewed journal publications, although many don’t necessarily...

  39. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...recent conversations with three lab heads have revealed that a combination of hard work, determination...How do you manage work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and hopefully...Scientists Maintain a Good Work-Life Balance? Read the Previous Posts in this Series Advice for Choosing a Research...

  40. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...be completed and reviewed. The timeline therefore will be as follows:  Request review: 1–2 days Prep and...µL (5 mL).  Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...
Showing: 201 - 240 of 754 results