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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 210 results for: cycs

Showing: 181 - 210 of 210 results

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander...

  3. AAV ddPCR Titration

    Type
    Protocol
    ...sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters. Cycling Step Temperature...Droplet Digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1...Temperature (°C) Time (min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing...

  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters: Cycling Step Temperature...Droplet digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1...Temperature (°C) Time (min) Ramp Rate (°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 ...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... heating and cooling (denaturing and annealing) cycle. The process became automated with the discovery...polymerase can withstand many heating and cooling cycles, which would denature DNA polymerases from other...a few nanograms of template DNA through several cycles of denaturation, annealing, and synthesis. After... 2 minutes at 72°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. Run 2 μL...

  6. Kit Free RNA Extraction

    Type
    Protocol
    ...avoid multiple freeze-thaw cycles. Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample...

  7. Genetic Code Expansion

    Type
    Collection
    ...122650 Mm-PylRS-AF/Pyl-tRNACUA PylRS M. mazei trans-cyclooct- 2-ene-lysine (TCOK) Mammalian TAG Howard Hang...

  9. Antibody Guide

    Type
    Collection
    ...least 10 µL. Antibodies cannot tolerate freeze-thaw cycles. Here are a few tips for storing your antibody:...

  10. Fluorescence Titering Assay

    Type
    Protocol
    ...lentiviral supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells into each well of ...and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and aliquoting...

  11. Colony Formation Titering Assay

    Type
    Protocol
    ...supernatants be subjected to multiple freeze-thaw cycles. This protocol outlines the seeding of the cells...and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and aliquoting...

  12. Pouring LB Agar Plates

    Type
    Protocol
    .../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...from the autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the...

  13. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...or -80°C for long-term storage. TIP: Freeze/thaw cycles decrease the efficiency of the virus, so Addgene...into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting Target Cells Lentiviral...

  17. Ligation Independent Cloning

    Type
    Protocol
    ...product is then repaired during the replication cycle. Empty vectors for LIC typically employ type II ...

  21. Sequencing Primers

    Type
    Guide
    ...Cre recombinase, reverse primer CYC1 GCGTGAATGTAAGCGTGAC (Invitrogen) CYC1 transcription termination signal...CGCAACGATCTGGTAAACAC (Invitrogen) OpIE2 promoter, forward primer pACYC-F TGAAGTCAGCCCCATACGAT p15A origin, forward primer... primer Tet-R GGCGAGTTTACGGGTTGTTA 5' end of tetracycline resistance gene, reverse primer TK-pA-R TTGTCTCCTTCCGTGTTTCA...

  22. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...replicate in the host cell and complete the lytic cycle. AAVs are therefore non-pathogenic and generally... viruses, wild-type AAVs cannot enter the lytic cycle, and instead establish latent infections by integrating...actively causing symptoms or replicating. Lytic cycle Viral replication process where the virus uses the...

  23. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...genomes into hosts in order to continue their lifecycle and replicate. This feature can be harnessed for...Gamma-retroviral Packaging Systems The Retroviral Lifecycle Types of Viruses Viral Vector Elements More Viral...

  24. Lentiviral Vector Guide

    Type
    Guide
    ...genomes into hosts in order to continue their lifecycle and replicate. This feature can be harnessed for...Lentiviral Plasmid FAQs Answered The Retroviral Lifecycle More Viral Vectors 101 topics More Lentiviral ...

  25. CRISPR Guide

    Type
    Guide
    ...efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 ... wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries ...

  26. Promoters

    Type
    Guide
    ...from phospholycerate kinase gene TRE Inducible Tetracycline response element promoter U6 Constitutive Human...

  27. Chemogenetics Guide

    Type
    Guide
    ...inhibit neuronal signaling by inhibiting adenylate cyclase and downstream cAMP production. There are currently...

  28. Optogenetics Guide

    Type
    Guide
    ...nm) BLUF domains bPAC Light-activated adenylyl cyclase from Beggiatoa sp. for light-induced cAMP modulation...

  29. Molecular Biology Reference

    Type
    Guide
    .../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL *Note: Carbenicillin can be...

  30. Antibody Guide

    Type
    Guide
    ...least 10 µL. Antibodies cannot tolerate freeze-thaw cycles. Here are a few tips for storing your antibody:...
Showing: 181 - 210 of 210 results