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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 40 results for: PAM

Showing: 1 - 20 of 40 results

  1. CRISPR Guide

    Type
    Collection
    ...enzymes with altered PAM specificities are available, known as PAM-flexible or PAMless Cas9s. SpCas9-derived...adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG) serves as a binding signal for...Increasing Cas9 PAM flexibility CRISPR systems designed for more precise edits require PAM sequences in ...region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant throughout the human...= C or T SpRYc - SpRY PAMs plus NNG; a chimeric version of SpRY Though these PAM-flexible Cas9s are useful...TTTV (V = A, C, or G) PAM sites. Engineered versions of Cas12a recognize different PAM variants, with one...of Cas Enzymes Cas Enzymes and PAM Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes...

  2. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... may only be compatible with a single species or PAM binding variant of Cas9. For instance, wild-type ...used with targets that are upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence...systems with wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with alternative PAM sites . Cpf1 , another CRISPR nuclease, recognizes a 5’-TTN PAM, improving targeting... sequence known as a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and...it contains a special Protospacer Adjacent Motif (PAM) directly after where the crRNA would bind. (5) After...stranded DNA target and cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after...bacterial genomes, are highly diverse, with varying PAM sequences and number/types of Cas proteins. Makarova...packaged in AAV. Cas9 orthologs also have distinct PAM sites that do not cross react, allowing multiple ...2015. Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369...

  4. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription...

  5. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell...sequence identity to the spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae ...

  6. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...to uridine within a small editing window near the PAM site. Uridine is subsequently converted to thymidine... to work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide...

  7. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) ...

  8. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49410 pCFD3-dU6:3gRNA dU6:3 BbsI Injection...

  9. CRISPR Plasmids - Yeast

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1 HindIII...

  10. Plan Your Experiment

    Type
    Collection
    ... no PAM site close to your desired edit site. Consider alternative Cas9 enzymes with different PAM sequences...activity within a very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions...sequences or Cas9 enzymes with flexible PAM sites . Base Editing : Target the site of your desired edit...their functions within a limited window from the PAM sequence, and base editor variants often have different...

  11. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s protospacer sequence and PAM fall... “NGG” sequence (“protospacer adjacent motif” or PAM) at the genomic recognition site. NOTE: Figure 1 ...independent of orientation of the protospacer sequence/PAM relative to the top or bottom strand. Determine the...

  12. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...sequence identity to the spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved...

  13. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7 BsaI In...

  14. CRISPR Plasmids - Parasites

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...and need to be followed on the 3' end by a 3bp NGG PAM sequence. We have found that adding an additional...and need to be followed on the 3' end by a 3bp NGG PAM sequence. 3. SpCas9 alone: This plasmid contains ...need to be followed on the 3' end by NGRRT or NGRRN PAM sequences. We have found that adding an additional...

  18. CRISPR Plasmids - Plants

    Type
    Collection
    ...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell...

  19. Validated gRNA Sequences

    Type
    Collection
    ... may only be compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must...used with targets that are upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA ...
Showing: 1 - 20 of 40 results