...Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein SHC SHC1 SHC2 ...

...pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol Syst Biol 2023...Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al., Nature Methods 2022) is adapted...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: A multi-purpose, regenerable, proteome-scale...in a new window) rEcoli XpS and a new pSerOTS described in: System-wide optimization of an orthogonal ..., 551 KB) Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria...in a new window) Improved Reagents (ca. 2015) described in: A flexible codon in genomically recoded Escherichia...Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Enhanced phosphoserine insertion during Escherichia...

...expression plasmids. Large collections or kits are described below and not included in this table. For a more...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic ...

...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...

... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with...Constitutively active Cas9 mouse Available plasmids are described below: 61408 : Targeting vector for the mouse ... backbone plasmid for cloning new targets are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA...present in the mouse genome. These plasmids are described here. #60227 - AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR...

...production of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization... the genomic recognition site. NOTE: Figure 1 describes possible deletion strategies for genes and non-coding...deletion. For this approach, the internal primers described in step 2.1 may be omitted. CRISPR Cloning Anneal...plasmids by electroporation 4 . This protocol is described in detail for murine erythroleukemia (MEL) cells...

...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the...

...cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated...Engineering newsgroup here . Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression...

...regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the target site sequence...non-model invertebrate species. These plasmids are described in: Genome engineering of Drosophila with the ...

...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...For more information, see the bioRxiv preprint describing ICE . Deep Sequencing Data Analysis CRISPResso...

...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science ...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...

...the particular conditions of the experiment as described in the associated article (listed below by PubMed...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page...

...and educational use. Links to additional pages describing these reagents are provided below: Zinc Finger...

...Daniel Voytas Lab plasmids The Joung Lab recently described a rapid and robust protocol for constructing TALE...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...