...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...Calcium Ratiometric calcium biosensors from nested reporters of green- and orange-emitting proteins Ratiometric...

...Tagging Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification...expression at a specific level. Return to Top Reporter Plasmids Reporter plasmids can be used to detect events...they activate expression of the reporter gene. Plasmid ID Promoter Reporter PI Purpose pRU Series Various...of promoter probe vectors incorporating fluorescent and chromogenic reporter proteins for studying gene...Purification Controlled Expression Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular...promoters, their associated transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum...bacterium. Plasmids containing easily measurable reporter genes (eg. LacZ or Fluorescent Proteins ) under...

...Multiple Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, ...transcriptional regulation and bioluminescent reporters. Plasmid...Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase is the enzyme responsible... common choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors...-lives, making them useful as transcriptional reporters. However, they are not secreted, so cells must...CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that contains an intron in the middle of the Firefly.... pKC-4.04, pKC-4.06 : Nonsense-mediated decay reporter and control plasmid utilizing Firefly luciferase...

... the codon), the amino acid from the tRNA is incorporated into the growing peptide chain. To expand the...aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid ...the cells, the non-canonical amino acid can be incorporated using the existing protein translation machinery...translation machinery are needed in order to incorporate a non-canonical amino acid into the protein of... the place where the new amino acid would be incorporated. Other options, such a 4-base pair codons, have... are true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...modified to enhance non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all ...

...sites AAV Uchida Cre Reporters and Tools In this subtype of loxP plasmids, reporter genes indicate which...Mammalian Pelczar 62732 Cre Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References Background...activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated Cre: The promoter...Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized...recombination in E. coli , deleting the floxed portion of a construct during the cloning process). VCre...HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-EF1a-N-CretrcintG...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...Plasmid Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials...pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase...destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...

... 10 6 cells in 100 μl of electroporation solution and add to electroporation cuvette. Add 5 μg of each...construct. Electroporate cells with 250 volts for 5 msec in a 2 mm cuvette using an electroporation system...of culture media after electroporation. Minimize the time between electroporation and transferring the ...involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described in detail for...transfection conditions for each cell line with a reporter construct to ensure robust plasmid delivery before...Li D, Li J. Cell Stem Cell . 2013. PubMed . Electroporation: theory and methods, perspectives for drug ...

...Collection The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...The vast majority of cases (90-95%) of ALS are sporadic, having no prior family history. A small percentage...specific known cause) nature of the disease, a small portion of cases can be attributed to known genetic factors... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell...This collection highlights plasmids created with support from the BRAIN Initiative. Jackson Laboratory (...a new window) . This resource was created with support from the Chan Zuckerberg Initiative (Link opens...

...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...Plasmids ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials...General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably detect ...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ...

...genome-wide gRNA libraries. PubMed PMID 28348817 . CRISPOR This tool helps design (10 different prediction...sequence and checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans...regulation (repression and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( ... or a sequence text file you upload. Currently supports: Human, mouse, rat, marmoset, pig, chicken, frog...thermophilus or N. meningitidis Cas9 PAMs. Currently supports: mouse and human. CRISPR Optimal Target Finder...

...Nuclear Export Sequence mTurquoise2 Dorus Gadella 85062 pmScarlet-i_NES_C1 Non-nucleus Nuclear Export Sequence...Nuclear Export Sequence mScarlet-H Dorus Gadella 85060 pmScarlet_NES_C1 Non-nucleus Nuclear Export Sequence...protein is often tied to its function, so it's important to determine where your protein of interest resides...2XG4S-mCer3 Mitochondria COX IV-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3...2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065... and adhesions between animal cells. They are important for holding cell structures together and reducing...translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in vesicles to be packaged...

...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI CACNA1A GFP U6, Cbh Spinocerebellar...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1..._Paragranulin-no linker GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176918 pcDNA3.1...pcDNA3.1(+)_Granulin 1+linker2 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176919 pcDNA3.1...

... has developed several tools useful for the incorporation phosphoserine into your gene of interest....University changed the way researchers can explore important questions surrounding serine phosphorylation by...in place of the popular SepOTSλ ( 68292 ). An important technical note: pSerOTS-C1* ( 188537 ) is a ROP...able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...phosphosite library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain...188531 ; 188532 ; 188533 ; 188534 ; 188535 ). Importantly, phosphorylation positional isomers are allocated...

...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...may alter protein function when they occur in important protein domains. For gene editing experiments ... addition to off-target activity , it is also important to consider factors that maximize cleavage of ...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...

...disorders. The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...and antibodies created with support from the BRAIN Initiative below. Support has been identified by depositors...The BRAIN Initiative supports the development of biomolecular tools for neuroscience research. Browse...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal...128485-AAV9 pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin ...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage... HDR efficiency. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...applications where orientation of the insert is important. Type V-K CASTs appear to exhibit almost unidirectional...

...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think...which are bacterial proteins that bind to the Fc portion of an antibody. However, they are not suitable ... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...

... cluster in the MBD and NID demonstrating the importance of binding to methylated DNA and the recruitment...syndrome at the molecular level. They are also an important tool for developing therapeutic strategies to ...MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) PMID: 35148843...opens in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in ...Protocols.io - protocol sharing site Funding Opportunities (Link opens in a new window) Rett Syndrome Research... purposeful hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens...

...collection differ based on some important criteria, and it is important to know which library best...mind: CRISPR screening experiments require electroporation to amplify the pooled library and the use of...best suits your particular experiment. Some important variables include: Type of genetic modification - ...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...Knockout Human Superti-Furga 3rd 6 2,609 Human Transporter Knockout Libraries 213695 221409 221410 Knockout...

...NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature cell targeting...recognition sequences enables directional control of the portion of the ORF that is sandwiched between the sites...working with INTRSECT recombinases, viruses, and important controls to consider as part of experimental design..., Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission... a new window) Mingote S, Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections...

...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified...agreement No 777372. This Joint Undertaking receives support from the European Union's Horizon 2020 research...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...knockout library targeting human transmembrane transporters. Feedback Please send your feedback to the Resolute...

...Addgene assists importation by including thorough shipping documents and following import procedure, it ...it is the responsibility of the importer to pay for any additional customs fees or taxes, and to complete... that might be required by your country for the import of biological research materials. Please be aware...

...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49409 pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in...transcription Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, ...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases... making targeted mutations in zebrafish. With support from the NIH and guidance from the academic community...Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger arrays listed show transcriptional...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...

...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory...

...(sodium/bile acid cotransporter family), member 2 ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette...grouped into tables based on the Gene Lists from Immport . Click the links below to go to the gene table...perforin and granzyme B, two enzymes that create pores within an infected cell membrane, triggering cell...polypeptide FCRG FCGRT Fc fragment of IgG, receptor, transporter, alpha FCRN, alpha-chain HFE hemochromatosis ...FLJ41500, PSF1, RING4, TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP...ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin ADIPOR1 adiponectin receptor 1 ACDCR1, CGI-45, CGI45, FLJ25385... FLJ25385, FLJ42464, PAQR1, TESBP1A ADIPOR2 adiponectin receptor 2 ACDCR2, FLJ21432, MGC4640, PAQR2 ADM...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...

...Selectable markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome...stable cell lines in which the plasmid is incorporated into the host DNA. For mammalian cells, you ... AdEasy®-1 plasmid Back to Top Reporters, shRNA expression, transgenics and genome ...promoter strength pBV-Luc - Luciferase reporter plasmid with very low basal activity shRNA/RNAi...

...Browse plasmids supporting the evaluation of a diverse set of novel Alzheimer's disease targets....TREAT-AD centers are focused on diversifying the portfolio of drug targets in Alzheimer’s disease (AD) by...molecules for prioritized targets that are valued opportunities for further translational investment. All resources...

...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

...research opportunities to their students and other members of the MCC community. They support educators...local hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional...

...identify novel interactions or pathways that are important in cellular recognition processes. Laboratories...Babesia microti blood stage proteins P. falciparum sporozoite cell surface and secreted proteins Mouse sperm...protein interaction network and its resolution by spatiotemporal expression profiling. Martin S, Söllner C, ...

...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC)... elegans. - Jane Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans...

...114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding... from the Allen Institute for Cell Science data portal (Link opens in a new window) , including: data ...

...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...This DNA is then used as a template to amplify a portion of the intended lentiviral insert. During this ...

...Bullock and Port pCFD2-dU6:2gRNA 49409 Fly BbsI none S. pyogenes Virmilion Bullock and Port pDCC6 59985...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA 45946 Drosophila BbsI none S. pyogenes...

...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...

...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

...virus). In the absence of tetracycline, the tetR portion of tTA will bind these tetO sequences and the activation...identify which amino acid residues of tetR were important for tetracycline-dependent repression. Mutating...shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping...

...plasmid webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters...MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301 cre...intron-lacZ/NLS Additional Resources References Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks...

...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...

...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...

...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster...

...Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and...

...accessibility in research. For each antibody, we provide important details including the protein and plasmid sequence...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....

...Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether...PSAM is activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types...

...Where can I find cloning resources or scientific support? Please see our Addgene Protocols for resources... may find helpful. Our Scientific and Customer Support Teams are also available to provide assistance ...

...Resources Addgene's Data Hub Curated technical reports from the research community demonstrating AAV performance...plasmid. Distribution of the original plasmid is important because it is the actual material being referenced...

...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...