...generate virus (right), similar to how natural viruses can be delivered to a host and generate virus (left...more viruses. When a virus infects a host cell, its genome directs the production of more viruses. However...Type of Virus Should I Use? Use this guide to learn more about the different types of viruses that are...genome. Adeno-associated Virus (AAV) Plasmids | Guide Adeno-associated viruses (AAVs) are commonly used... most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction... DNA for use with virus-mediated delivery has many advantages for research. Viruses facilitate the delivery... they give rise to viruses with modified viral genomes. Naturally occurring viruses harbor genetic material...

...for rabies virus-based monosynaptic tracing. These AAV can be used with modified rabies virus to perform...that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect any neighboring...envelope protein of (EnvA) of leukosis virus. EnvA can direct virus infection specifically into cells that...neuronal tracing, to be used with modified rabies virus. Viral...tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal Tracing ID...Can be used to complement deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...can be used to complement deletion-mutant rabies virus AAV1 Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G...

...components of one virus (such as HIV, murine leukemia virus, and vesicular stomatitis virus G) are packaged...the study of the live virus. Alternatively, it is possible to generate pseudovirus, where the structural...suitable for generating pseudotyped virus for SARS-CoV-2 and other coronaviruses using appropriate packaging ... Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (184 KB) Addgene Blog Posts Viral ...-19 - Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly...packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope...envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that express ACE2...

...Plasmids Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors...101 eBook Adenoviruses are a class of medium-sized, non-enveloped, double-stranded DNA viruses known to...levels of transgene expression. Additionally, these viruses are able to target a broad range of dividing and... Bunz 16402 pShuttle Shuttle For production of viruses containing transgene under a chosen promoter Vogelstein...Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter Vogelstein...pAdTrack Shuttle For production of GFP-trackable viruses containing transgene under a chosen promoter Vogelstein...pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene under CMV promoter Vogelstein...

...Adeno-associated viruses (AAV) are small viruses originally discovered as contaminants of adenovirus stocks. One... presence of another "helper" virus, such as adenovirus or herpes virus, in order to propagate. This is...Viral Plasmids Adeno-associated Virus (AAV) Plasmids Adeno-associated Virus (AAV) Plasmids Additional Resources... requirement has been circumvented with “helper-virus free systems,” which enable the production of infectious...infectious AAV particles without the use of a helper virus. Instead, specific gene products can be provided...infectious AAV particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding... Helper plasmid for AAV packaging, expressing adenovirus E4, E2A and VA Wilson RepCap Plasmids for Serotypes...

...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus (WNV) ... your microbe of interest, including bacteria, viruses, protozoa, fungi, and more. Plasmid...range of microbial fields, including bacteria, viruses, parasites such as protozoa, and fungi. Find plasmids...complementation constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each... the predicted open reading frames for vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden...

... specific virus in order to generate high quality preparations. After production, all virus preps are ...Resources Lentivirus Production Titer Quality Control Resources Overview of Viral Production Viruses are generated...experiment using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient instructions...contamination. In the event of contamination, all of the virus produced in the line will be taken off-line and ...preps available from Addgene After receiving your virus from Addgene, read our detailed recipient instructions...instructions for next steps. Plan an experiment using our virus protocols . Browse our plasmid collection of popular...

...that was used to make the virus. For more details, see our FAQ on What is virus associated DNA, and why ...Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample of the ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

...100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with ... 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with ...millisecond resolution optogenetics. Cre-dependent virus Edward Boyden 108422-AAV5 pAAV-CAG-FLEX-Archon1-...pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter...pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter ...pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter...

...Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety Blog: Tips for Titering...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may require ...cells without a significant immune response. These viruses also integrate stably into the host genome, enabling...biosafety procedures. Additionally, because this virus integrates randomly into the host genome, it has...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms...

...Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and ...cell, the virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common...γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV...transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce dividing cells...breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate randomly into the host's genome, which...

...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...

...Background The Coronavirus Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the... elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like that...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent... Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity...mediating the entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes ...primes the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves... to the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine...

...catalog items. Virus-associated DNA Each viral vector service request will also include virus-associated ... the original plasmid that was used to make the virus. In order for Addgene to provide viral vector services... libraries. Viral preparation options include lentivirus and AAV. Viral...Order a high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV...

...165510 pOpen-DNA Topoisomerase 1B Vaccinia Virus Vaccinia Virus DNA Topoisomerase 1B 165515 pOpen-EcoRIM...165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated... pOpen-MMLV_RT (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH...

...Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin...Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar 28;90(8):4215-4231. PMID:...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

... controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository...guarantee viral service, but lets us know what viruses would be helpful to the scientific community.... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in ... viral preparation from the inventory below. Lentiviruses are distributed as purified viral particles ...

... Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome editing across... improved vector system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome ...

...transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral... or not you've efficiently generated infectious virus, plasmids expressing both the Cre and Flp recombinases... Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of ...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...

...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based...recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases 10–14 . Figure...principles for working with INTRSECT recombinases, viruses, and important controls to consider as part of ...

...Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids ...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Text...

... expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both...improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies...vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...your gene of interest pFastbac1 Nterminal GST - Baculovirus expression vector with N-terminal GST pGCS-N5...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome Modifications...

...other prime editors use the Moloney murine leukemia virus (M-MLV) reverse transcriptase. Engineering of the...

...for openly shared communication: Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

... Plasmids Additional Resources Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...

...to mutagens like radiation or viruses (e.g., HPV, the human papillomavirus). These mutations interfere ...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...transcriptional activation domain from HSV (herpes simplex virus). In the absence of tetracycline, the tetR portion...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

...luciferase; GFP is expressed if cells are infected with virus Christopher Buck 98580 pLX304 Luciferase-V5 blast...

...C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus (KSHV) Cow ( B. taurus ) Library Size Genome-wide...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...

...2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID...

... of Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus (CMV, PX601 [#61591] ) or liver-specific tyroxine...

...E, Levi BP, Ting JT, Zeng H, Tasic B. Enhancer viruses for combinatorial cell-subclass-specific labeling...

...responses of the immune system upon infection with virus and, like other cytokines, enable communication ...TCF16 CR2 complement component (3d/Epstein Barr virus) receptor 2 C3DR, CD21, SLEB9 FCGR2B Fc fragment... 1 (Xenopus laevis) DKK-1, SK EBI3 Epstein-Barr virus induced 3 IL27B EDN1 endothelin 1 ET1, HDLCQ7 EDN2...fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog) HBGF...receptor GPR38, MTLR1 MPL myeloproliferative leukemia virus oncogene C-MPL, CD110, MPLV, TPOR MSTN myostatin...described. Symbol Name Synonyms EBI3 Epstein-Barr Virus Induced 3 IL35B, IL27B, IL35 subunit IL10RA interleukin...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

... be efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9...editing method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the...H840A nickase and M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase) for "search and replace" ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...

...guarantee viral service, but lets us know what viruses would be helpful to the scientific community. Penn...

...11919 pBS448 RSV-GFPcre Cre-GFP fusion Rous sarcoma virus (RSV) LTR/promoter Mammalian Sauer 11920 pBS500 ...

...Delivery Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc.... Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog, Oct4, ...Science. 2007 Dec 21;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human...Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector .... Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector ...Stem Cells Dev. 2010 Aug;19(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible...Pluripotency. Cell. 2015 Jul 16;162(2):412-24. Mikkelsen Lentivirus Human Expression of human Klf4, Oct4, c-Myc, ...

...or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags...collection at Addgene. Addgene's COVID-19 and Coronavirus Plasmids & Resources page outlines our entire...

...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...

...analyses to pinpoint genes linked to parasite virulence, transmission, and development. This collection...

...Hexahistidine tag with Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine...

...dcas9 gene under the control of Murine Stem Cell retroVirus LTR promoter. The second plasmid ( pgRNA_humanized...