Skip to main content
Addgene
Showing: 1 - 50 of 1257 results

  2. Mucolipin1-pEGFP C3

    Plasmid
    #62960
    Purpose
    full length human Mucolipin-1 cloned into pEGFP C3
    Depositor
    Paul Luzio
    Article
    Pryor et al Traffic. 2006 Oct;7(10):1388-98.
    Insert
    Mucolipin-1 (MCOLN1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mucolipin-1 has 1 silent mutation compared to the…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid
    #192835
    Purpose
    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells
    Depositor
    Karin Reinisch
    Article
    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070
    Insert
    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    Use
    Tags
    3xFLAG-PreScission cleavage site and 6xhis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192835-plasmid-map-sequence-id-379957

    Enlarge

  27. MmSphk1

    Plasmid
    #118599
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus sphingosine kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    118599-plasmid-map-sequence-id-234126

    Enlarge

  28. HsSPHK1

    Plasmid
    #118091
    Purpose
    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    118091-plasmid-map-sequence-id-234124

    Enlarge

  29. HsCERK

    Plasmid
    #118600
    Purpose
    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens ceramide kinase
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Feb. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    118600-plasmid-map-sequence-id-234128

    Enlarge

  34. MmSphk2

    Plasmid
    #118598
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    118598-plasmid-map-sequence-id-236037

    Enlarge

  35. HsSPHK2

    Plasmid
    #118092
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    118092-plasmid-map-sequence-id-234125

    Enlarge

  45. pCW57.1_SPTLC1_WT

    Plasmid
    #181920
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181920-plasmid-map-sequence-id-361674

    Enlarge

  47. PLC-TIR-GFP

    Plasmid
    #52736
    Purpose
    Retroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP and PLCd1 Lipid binding
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1-85
    Promoter
    Available since
    June 18, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 50 of 1257 results