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Showing: 361 - 400 of 19647 results
  1. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197026
    Purpose
    Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized enOsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R
    Promoter
    CBh, CMV, hU6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized enRhCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197024
    Purpose
    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized OsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197025
    Purpose
    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized RhCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

    Plasmid
    #236148
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site
    Depositor
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the UTRN promoter region
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

    Plasmid
    #190079
    Purpose
    express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
    Depositor
    Insert
    ACE2 (TMPRSS2 Synthetic, Rhinolophus alcyone and Rhinolophus ferrumequinum)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    none
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid
    #49080
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S, C820S, C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
  11. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

    Plasmid
    #214138
    Purpose
    Insect cell expression of human 20S CP alpha-subunits
    Depositor
    Inserts
    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    PSMA5 (PSMA5 Human)
    PSMA6 (PSMA6 Human)
    PSMA7 (PSMA7 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

    Plasmid
    #176589
    Purpose
    Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer
    Depositor
    Insert
    CRISPR-associate IscB (Chesapeake Bay) locus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Truncated CRISPR array to two direct repeats, rep…
    Promoter
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid
    #172501
    Purpose
    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers
    Insert
    DisCas7-11 full locus with Golden gate acceptor site for spacer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid
    #172908
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    Has Service
    AAV1
    Insert
    Ace2N-4AA-mNeon-ST A122D
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid
    #197031
    Purpose
    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter
    Depositor
    Insert
    humanized denOsCas12f1-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  16. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid
    #214140
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7
    Depositor
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid
    #214139
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2
    Depositor
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. AiP14002 - pAAV-AiE0064h-spacer-AiE0022h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4002)

    Plasmid
    #230389
    Purpose
    AiE0064h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  19. EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236177
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/IKZF1 sites
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. EW373 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236175
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF250 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. EW374 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid
    #236176
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and GFP-NZF dummy transcription factor
    Depositor
    Inserts
    EGFP-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF35 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176459
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Insert
    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176460
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Insert
    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

    Plasmid
    #236173
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor
    Depositor
    Inserts
    ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF35 sites
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

    Plasmid
    #236172
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor
    Depositor
    Inserts
    ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/ZNF250 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid
    #236174
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)
    Depositor
    Inserts
    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and four ZNF35/IKZF1 sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
  28. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Insert
    EJ7-GFP variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Insert
    4-μHOM variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 361 - 400 of 19647 results