We narrowed to 9,536 results for: pho;

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SYKA-c020

Plasmid

#175489

Purpose

Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

Depositor

Opher Gileadi

Article

Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

Insert

SYK (SYK Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Stat3-SH2

Plasmid

#46513

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat3 (STAT3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 584-700

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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p21-WT-MEFV

Plasmid

#134702

Purpose

Lentiviral expression of human MEFV after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

WT

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag PGC1 T177A S538A

Plasmid

#18093

Depositor

Bruce Spiegelman

Article

Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

Insert

PGC-1a (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

T177A S538A

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCMX-PL1-WDR5

Plasmid

#59970

Purpose

Mammalian Expression of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-WDR5

Plasmid

#59969

Purpose

Bacterial Expression GST-WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pRSV-CaMKIV(1-313)

Plasmid

#45063

Purpose

Expression of constit active CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV(1-313) (Camk4 Mouse)

Expression

Mammalian

Mutation

truncated at amino acid 313*

Promoter

RSV

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (396-492)

Plasmid

#86755

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 396-492

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (181-413)

Plasmid

#86754

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 181-413

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (201-492)

Plasmid

#86751

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

aa 201-492

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-R438E

Plasmid

#91984

Purpose

Expresses FLAG-HA-AGO2 (R438E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

R438E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Slp76-SH2

Plasmid

#46503

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slp76 (LCP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-533

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YD EGFP

Plasmid

#187264

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

Plasmid

#69842

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M-S285A-T289A

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Fer-SH2

Plasmid

#46425

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fer (FER Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 450-558

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C

Plasmid

#171247

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys (AR-W741C)

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S222TAG

Plasmid

#68302

Purpose

N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Lyn-SH2

Plasmid

#46452

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lyn (LYN Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 106-213

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Hck-SH2

Plasmid

#46445

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Hck (HCK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 134-254

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Stat1-SH2

Plasmid

#46511

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat1 (STAT1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 488-683

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-VSV

Plasmid

#139346

Purpose

Expression of kinase-dead TYK2-K930R protein

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727A-STAT3-3xFlag

Plasmid

#99262

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727A)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727A (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

S727A

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-S727D-STAT3-3xFlag

Plasmid

#99263

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (S727D)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - S727D (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

S727D

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Bmx-SH2

Plasmid

#46409

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Bmx (BMX Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 286-400

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCSGFPnERT(#205)

Plasmid

#184065

Purpose

cyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

eGFP-nls-ERT2

Use

Synthetic Biology

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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