We narrowed to 4,410 results for: BRE

1. TET-pLKO.1 PURO shSMAD4 #2

   Plasmid

   #83091

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human SMAD4

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shSMAD4 (SMAD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

   Plasmid

   #125225

   Purpose

   Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-fCR-GFP

   Plasmid

   #22911

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   fugu calretinin promoter driving GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

4. p045

   Plasmid

   #70057

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with pDC184

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   C2AB-linker-SNAP-25_C
   SNAP-25_N
   

   Use

   Tags

   No tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICE-EGFP-FLAG-Ku70siR-WT

   Plasmid

   #46961

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   human Ku70 (XRCC6 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Tet

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJCC_075 AsCas12a D908A

   Plasmid

   #171668

   Purpose

   For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a D908A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed aspartate 908 to alanine

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

   Plasmid

   #111260

   Purpose

   mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   DsRed-Express2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pc

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSW002-Pc-TorA(sp)

   Plasmid

   #111253

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorA signal peptide
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pc

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5FRT/TO-p97wt-mycStrep

   Plasmid

   #31837

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Use

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

10. dCas9-NLS-gs10-cGR2176

    Plasmid

    #188258

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cGR2176
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIRESpuro2-UBXD1-3x HA

    Plasmid

    #31836

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    UBXD1 (UBXN6 Human)
    

    Use

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

    Plasmid

    #125230

    Purpose

    For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
    

    Use

    Lentiviral

    Tags

    HA,Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5FRT/TO-p97-EQ-mycStrep

    Plasmid

    #31838

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    E578Q

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

14. ∆C' terminal-DPYSL2-pLJC2

    Plasmid

    #189867

    Purpose

    Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

    Insert

    ΔC-DPYSL2
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    deleted the C'terminus of DPYSL2 (491-572 aa)

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-WRAP53beta

    Plasmid

    #64676

    Purpose

    expresses Wild type WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

17. 373_MLH1 in pmiRGlo

    Plasmid

    #78133

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    MLH1 (MLH1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Psicheck2 Sox4 full length 3'UTR

    Plasmid

    #26989

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 Full length 3' UTR (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pEF1a-2xSV40_NLS-CeNLuc

    Plasmid

    #135952

    Purpose

    Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Tags

    2xSV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF1a-T2-CeNLuc

    Plasmid

    #135948

    Purpose

    Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge