We narrowed to 2,218 results for: dcas9

1. AAVS1-iSAM

   Plasmid

   #211495

   Purpose

   AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

   Depositor

   Lesley Forrester, Antonella Fidanza

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   UniSAM insert
   TET-ON
   Neo Resistance
   Insulator
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-mCherry Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, NA, Splicing acceptor, and TREGV promoter

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

   Plasmid

   #99690

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

   Plasmid

   #140196

   Purpose

   Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dSpCas9-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta) , NLS

   Expression

   Mutation

   Promoter

   PgpdA and PtrpC

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a FLAG-CHMP2B

   Plasmid

   #232012

   Purpose

   Expression of FLAG-tagged CHMP2B

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a FLAG-CHMP2B-d55-96

   Plasmid

   #232013

   Purpose

   Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   CHMP2B (CHMP2B Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Δ55-96

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a mNeonGreen-3K-B1-10-IRES-mKate2-2A-PuroR

   Plasmid

   #215377

   Purpose

   Lentiviral expression of a cytosolic-localized split mNeonGreen variant that enables efficient complementation when the missing beta strand is present. mKate2 is present as a transduction control.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   mNeonGreen-3K-1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. synapsin-hIRβ-mCherry

   Plasmid

   #228449

   Purpose

   Expressing a truncated, constitutively active human insulin receptor (IRβ) with mCherry in rat primary hippocampal neurons

   Depositor

   Olivier Thibault

   Article

   Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

   Insert

   human Insulin Receptor beta subunit (INSR Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   neuron-specific synapsin promote

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYTP MYRF sg1

   Plasmid

   #88857

   Purpose

   pAC154-dual-dCas9VP160-sgExpression with MYRF sg1 cloned into BbsI site

   Depositor

   Mahmoud Pouladi

   Insert

   dCas9VP160 and MYRF sg1 gRNA (MYRF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYTP MYRF sg2

    Plasmid

    #88858

    Purpose

    pAC154-dual-dCas9VP160-sgExpression with MYRF sg2 cloned into BbsI site

    Depositor

    Mahmoud Pouladi

    Insert

    dCas9VP160 and MYRF sg2 gRNA (MYRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYTP MYRF sg3

    Plasmid

    #88859

    Purpose

    pAC154-dual-dCas9VP160-sgExpression with MYRF sg3 cloned into BbsI site

    Depositor

    Mahmoud Pouladi

    Insert

    dCas9VP160 and MYRF sg3 gRNA (MYRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYTP MYRF sg4

    Plasmid

    #88860

    Purpose

    pAC154-dual-dCas9VP160-sgExpression with MYRF sg4 cloned into BbsI site

    Depositor

    Mahmoud Pouladi

    Insert

    dCas9VP160 and MYRF sg4 gRNA (MYRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYTP MYRF sg5

    Plasmid

    #88861

    Purpose

    pAC154-dual-dCas9VP160-sgExpression with MYRF sg5 cloned into BbsI site

    Depositor

    Mahmoud Pouladi

    Insert

    dCas9VP160 and MYRF sg5 gRNA (MYRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYTP MYRF sg6

    Plasmid

    #88862

    Purpose

    pAC154-dual-dCas9VP160-sgExpression with MYRF sg6 cloned into BbsI site

    Depositor

    Mahmoud Pouladi

    Insert

    dCas9VP160 and MYRF sg6 gRNA (MYRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit

    #1000000079

    Purpose

    CRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.

    Depositor

    Thoru Pederson

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

16. p2T-CMV-ABE8e_dNRCH-BlastR

    Plasmid

    #232720

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    ABE8e-dCas9(NRCH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within Cas9 D10A H840A

    Promoter

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO5-SgHottip-GFP-CRISPRa

    Plasmid

    #134990

    Purpose

    dCas9-mediated activation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H840A,N863A

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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