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We narrowed to 25,285 results for: crispr

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Showing: 2041 - 2060 of 25285 results

  3. pCMV-PE2-P2A-hMLH1dn

    Plasmid
    #174827
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-hMLH1dn
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174827-plasmid-map-sequence-id-343635

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  4. Ezh2[FL]-dCas9

    Plasmid
    #100086
    Purpose
    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14056 (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[FL]-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    100086-plasmid-map-sequence-id-197550

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  5. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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  7. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  11. pC0079 RESCUE-S

    Plasmid
    #130662
    Purpose
    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dRanCas13b-ADAR2dd
    Expression
    Mammalian
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    130662-plasmid-map-sequence-id-255012

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  12. pC0078 RESCUE

    Plasmid
    #130661
    Purpose
    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dRanCas13b-ADAR2dd (RESCUE)
    Expression
    Mammalian
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    130661-plasmid-map-sequence-id-255014

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  17. pX330-EN479

    Plasmid
    #86234
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse rosa26 locus
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86234-plasmid-map-sequence-id-168051

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  18. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid
    #101204
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101204-plasmid-map-sequence-id-198050

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  19. PX389 Fluviicola taffensis Cas9

    Plasmid
    #68326
    Purpose
    Fluviicola taffensis Cas9
    Depositor
    Feng Zhang
    Insert
    FtCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68326-plasmid-map-sequence-id-119542

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  20. PX388 Bacteroides coprophilus Cas9

    Plasmid
    #68325
    Purpose
    Bacteroides coprophilus Cas9
    Depositor
    Feng Zhang
    Insert
    BcCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    68325-plasmid-map-sequence-id-125196

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Showing: 2041 - 2060 of 25285 results