Skip to main content

We narrowed to 13,416 results for: sequence

Showing: 2041 - 2060 of 13416 results
  1. pGZ173 (pFA6a-SL-MNase-kanMX6)

    Plasmid
    #70234
    Purpose
    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Insert
    Short linker and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Available Since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. TMED5-bio-His

    Plasmid
    #53338
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    TMED5 (TMED5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. A2M-bio-His

    Plasmid
    #53423
    Purpose
    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    A2M (A2M Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Insert
    mKate2
    Use
    Gateway entry vector
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

    Plasmid
    #110830
    Purpose
    Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.
    Depositor
    Insert
    AZGP1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    T2A and dTomato
    Promoter
    CMV
    Available Since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. AAV-CAG-dDIO-lck-smMyc-4x6T

    Plasmid
    #196421
    Purpose
    Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette
    Insert
    Lck-smMyc
    Use
    AAV; Dre/rox; astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 2041 - 2060 of 13416 results