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pBT2dR3-proB

Plasmid

#122595

Purpose

BE PACE selection, express T7 RNA polymerase with degron-GTCCA target

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

T7 RNA polymerase

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ17940-PHR-SFFV-NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Plasmid

#252646

Purpose

Human expression vector containing SFFV promoter, ABE8e fused to PYL1 and pH intein

Depositor

Stanley Qi

Article

Chen et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Use

Lentiviral

Promoter

SFFV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSL7458 (Fta RNA polymerase)

Plasmid

#254269

Purpose

Encodes E. coli codon-optimized rpoA, rpoB, rpoC, and rpoZ from Flagellimonas taeanensis driven by an inducible T7 promoter

Depositor

Samuel H. Sternberg

Article

Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-

Inserts

rpoA
rpoB
rpoC
rpoZ

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBT100.106b

Plasmid

#122601

Purpose

clone APOBEC1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

APOBEC1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABEmax

Plasmid

#119815

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDY1314_RADARS_Luciferase

Plasmid

#193191

Purpose

Express Gaussia Luciferase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

RADARS Luciferase

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FMRP_P451S

Plasmid

#198693

Purpose

Expression of human FMRP protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FMR1 (FMR1 Human)

Tags

MBP

Expression

Bacterial

Mutation

changed proline 451 to serine

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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p4053 HA-E6AP isoform I

Plasmid

#8657

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform I (UBE3A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pAJM.661

Plasmid

#108532

Purpose

TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TtgRAM + PTtg-YFP

Expression

Bacterial

Mutation

TtgR: R130H A144T A148V

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3L

Plasmid

#35523

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3L (DNMT3L Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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CBP-HAT-GST

Plasmid

#21093

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

GST

Expression

Bacterial

Mutation

HAT domain: aa 1196-1718

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pNuc-cis

Plasmid

#202796

Purpose

RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

Depositor

David Edgell

Article

Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

Insert

TevSpCas9, sgRNA cassette, oriT, CenArsHis

Expression

Bacterial

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-2xFLAG-Atoh1

Plasmid

#101734

Purpose

Expresses mouse Atoh1 with FLAG tag

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Tags

2x FLAG

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-GFP

Plasmid

#117242

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin

Plasmid

#84293

Purpose

Expression of wt Merlin

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-flx-fDIO-HA-Rpl10a-T2A-myc-hM3D(Gq)

Plasmid

#223654

Purpose

Expresses vTRAP and hM3D(Gq) in a FlpON-CreOFF dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

vTRAP-T2A-hM3D(Gq)

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE

Plasmid

#234895

Purpose

CAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tag

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

FRT(F3F5)-ChRmine3.3-p2A-oScarlet

Use

AAV

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAG551-MAP4-greenFAST

Plasmid

#160366

Purpose

Expresses MAP4-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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EK0208 CMV-TO-BFPmut-stop-t70587 (FLP-IN)

Plasmid

#191138

Purpose

Expression of Trigger 1 in 3' UTR of an inactive BFP in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mAIM2-Flag

Plasmid

#51537

Purpose

Express Flag-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAG552-MAP4-redFAST

Plasmid

#160367

Purpose

Expresses MAP4-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBT13-splitC

Plasmid

#122596

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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GWF058

Plasmid

#133609

Purpose

UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

Gal4DBD-NS3a, DNCR2-VPR

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-PSMA

Plasmid

#11524

Depositor

Pamela Bjorkman

Article

Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

Insert

prostate-specific membrane antigen (FOLH1 Human)

Tags

His

Expression

Insect

Mutation

There is a short N-terminal truncation of this pr…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-stop

Plasmid

#117243

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

Plasmid

#191140

Purpose

Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMM146

Plasmid

#127218

Purpose

BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, BBM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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