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pNX3c-PK9

Plasmid

#78370

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
natMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCAM10

Plasmid

#86483

Purpose

Expresses p12 and p9-mRFP1

Depositor

Pamela Silver

Article

Myhrvold et al Nat Commun. 2015 Nov 30;6:10039. doi: 10

Insert

p12, p9-mRFP1

Tags

mRFP1

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLEELA

Plasmid

#79752

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Marc Jakoby

Article

Jakoby et al FEBS Lett. 2004 Nov 19;577(3):528-34.

Type

Empty backbone

Expression

Plant

Promoter

2xPro35S

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

HA tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSGE_764_4xUAS_DOS_hkCP

Plasmid

#71172

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

764_4xUAS_DOS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pQFD

Plasmid

#48101

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Promoter

pLuxR

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-hygML

Plasmid

#73892

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

hygML

Expression

Bacterial and Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pN23-LIC-His-FLAG

Plasmid

#165478

Purpose

pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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369 pTol-lmo2:egfpDest

Plasmid

#73488

Purpose

Tol2 vector with lmo2 promoter upstream of EGFP, attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

lmo2 promoter:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c2-PK3

Plasmid

#78390

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
natCX

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRB33-GFP

Plasmid

#165483

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-GFP-TEV

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSGE_91_4xUAS_dCP

Plasmid

#71169

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

91_4xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b-PK9

Plasmid

#78363

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
hygMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

SYFP2

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3C06

Plasmid

#70979

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG9246

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3D11

Plasmid

#71007

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Acf1

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pYPQ132D-RZ-Lb

Plasmid

#134348

Purpose

Golden Gate entry vector; empty vector to clone the 2nd LbCas12a crRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-M

Plasmid

#1128

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

M (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal ts-lux

Plasmid

#1329

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

bacterial luciferase-V.h.MAV

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only