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pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRB306

Plasmid

#8666

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pHMMa.PH0999

Plasmid

#11453

Depositor

Sung-Hou Kim

Article

Du et al Biochemistry. 2001 Nov 27. 40(47):14166-

Insert

pyrazinamidase (PH0999 P. horikoshii)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
EML8543_pEML41003

Plasmid

#237864

Purpose

A suicide vector for Agrobacterium with kanamycin resistance.

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA1_105nt

Plasmid

#247075

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets pheS*_codon

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMut12_apo64C

Plasmid

#247072

Purpose

L-arabinose inducible rAPOBEC-XTEN64-CasC(Cas7)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

rAPOBEC-XTEN64-CasC-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA2I_LacZB1

Plasmid

#247076

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ B1 site

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pOP576

Plasmid

#85943

Purpose

Temperature sensitive plasmid based on pDK46. Expresses dCas9 from proD. Serves as a control for pOP587 - pOP594

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP549

Plasmid

#85941

Purpose

Bi-stable switch, similar to pOP512 but with a lambdaCI OR_1 operator in ptrc-2 promoter driving tetR.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Bi-stable switch

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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memGRKct-Venus

Plasmid

#234745

Purpose

Mammalian expression of plasma membrane anchored (GAP43) GRKct-Venus

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

memGRKct-Venus

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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mRuby2-MOA

Plasmid

#234747

Purpose

Mammalian expression of mitochondria marker mRuby2-MOA

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

mRuby2-MOA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCF

Plasmid

#216326

Purpose

PUlaA promoter driving expression of sfgfp

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSuccessor

Plasmid

#228391

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pConst23100-sfGFP__pLambda-DHFR

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDifferentiation

Plasmid

#228393

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pLambda-luxR+LVAtag__pLux-mRuby2

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-Butelase

Plasmid

#221848

Purpose

Expresses asparaginyl endopeptidase butelase

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGS275_6xHis-EGFP-CENP-T[1-242/3D (T11, T27, T85)]

Plasmid

#211891

Purpose

Bacterial expression of CENP-T[1-242/3D]

Depositor

Iain Cheeseman

Insert

CENP-T[1-242/3D] (CENPT Human)

Expression

Bacterial

Mutation

T11D, T27D, T85D

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_erbb2_gRNA171

Plasmid

#199385

Purpose

pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

erbb2 sgRNA 171

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-P C-FlagHA HPV16 E7 H73A

Plasmid

#191547

Purpose

Protein expression of HPV16 E7 H73A

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16

Use

Retroviral

Tags

FlagHA

Mutation

H73A

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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