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We narrowed to 11,705 results for: chia

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Showing: 5401 - 5450 of 11705 results

  8. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  9. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  32. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  33. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  34. pMAL_dstβL

    Plasmid
    #73805
    Purpose
    Plasmid for selection for insertion and homodimerization
    Depositor
    Sarel Fleishman
    Article
    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:
    Inserts
    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    Tags
    Part Of the dstbl chiemra, Part of the dsTbL chim…
    Expression
    Bacterial
    Promoter
    NaN and ToxR promotor
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pMAL_dstβL

    Plasmid
    #73805
    Purpose
    Plasmid for selection for insertion and homodimerization
    Depositor
    Sarel Fleishman
    Article
    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:
    Inserts
    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    Tags
    Part Of the dstbl chiemra, Part of the dsTbL chim…
    Expression
    Bacterial
    Promoter
    NaN and ToxR promotor
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  40. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  41. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  42. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  43. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  44. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  45. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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Showing: 5401 - 5450 of 11705 results