We narrowed to 3,340 results for: GAI

Showing: 501 - 550 of 3340 results

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pBAD-6xHis-BglII

Plasmid

#196654

Purpose

Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Type

Empty backbone

Tags

6xHis tag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDX_init containing Sb-loop

Plasmid

#132697

Purpose

pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop

Use

M13 phagmid vector

Promoter

lac

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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mKO-pBAD

Plasmid

#54735

Purpose

Localization: Protein Expression Vector , Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and mKO

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pBEVY-4420-mCherry

Plasmid

#218828

Purpose

Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

4-4-20 scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSW549 YFP/mVenus

Plasmid

#115984

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mVenus

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTmycTNRC6C

Plasmid

#19874

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pTAPmyc-T2A-tagRFP

Plasmid

#140278

Purpose

Backbone for making a mammalian expression vector of the protein of interest

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW596 - mTurq2

Plasmid

#115985

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mTurquoise2

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE/WT SP with 5'UTR

Plasmid

#89330

Purpose

Inducible expression of WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL719

Plasmid

#140389

Purpose

Expresses QacR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-QacR-T

Tags

6XHis

Expression

Bacterial

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL721

Plasmid

#140391

Purpose

Expresses TtgR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-TtgR-T

Tags

6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-CDC12(WT)

Plasmid

#1168

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Added N-terminal GFP to CDC12 in plasmid CDC12(WT…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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PSuperPuroRetro-1660s(hPot1)

Plasmid

#11136

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against Human Protection of Telomeres 1 (control) (POT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
S019

Plasmid

#174552

Purpose

pET-iBclxL-v3, His tag for affinity purification

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

iBclxL-v3, Bclxl inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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S330

Plasmid

#174553

Purpose

pET-iBcl2-v4, His tag for affinity purification

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

ibcl2-v4, bcl2 inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pVL93 - VP16

Plasmid

#116036

Purpose

entry vector for GreenGate cloning method, D module

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

VP16

Use

Unspecified

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU1A-myc-T2A-tagRFP

Plasmid

#140269

Purpose

Expresses truncated U1A in mammalian cells

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Truncated U1A

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWp9-5

Plasmid

#73856

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAWp9-4

Plasmid

#73855

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
tdTurboRFP-pBAD

Plasmid

#54857

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 576

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and tdTurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-R-GECO1.2

Plasmid

#45494

Purpose

Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

R-GECO1.2

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HaloTag-LacI-mHP1a

Plasmid

#232637

Purpose

lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha

Depositor

Karsten Rippe

Insert

HaloTag-LacI-mHP1a (Cbx5 Mouse, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBEVY-pT231 scFv-GFP

Plasmid

#218827

Purpose

Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

pT231 tau scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
2MLS-HyperNova/pcDNA3

Plasmid

#221457

Purpose

Expressing HyperNova in mitochondria

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDV5 containing Sb-loop

Plasmid

#132695

Purpose

pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-PML IV/pRK5

Plasmid

#59742

Purpose

Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

PML isoform IV (PML Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDESTmycQKI

Plasmid

#19870

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

quaking homolog, KH domain RNA binding (QKI Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
pME-Kaede

Plasmid

#132972

Purpose

full-length of photoconvertible kaede with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

Kaede

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHSE901

Plasmid

#91708

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-LacI

Plasmid

#183920

Purpose

Lac repressor labeled with GFP; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

GFP-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

SV2

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Synapsin-cyto-mRuby3-iATPSnFR1.0

Plasmid

#102557

Purpose

expresses cytoplasmic ATP sensor in neurons with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Ruby3

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW181 - GR-LhG4

Plasmid

#115983

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

GR-LhG4

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXSE901BG

Plasmid

#91714

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nme2Cas9_pMCSG7

Plasmid

#119925

Purpose

Nme2Cas9 bacterial expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS, Nucleoplasmin NLS, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-TetR-KRAB-IRES-BLAS

Plasmid

#55820

Purpose

retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

TetR-KRAB

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSb_init containing the MBP sybody Sb_MBP#1

Plasmid

#132699

Purpose

expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

sybody_MBP#1

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00029 (aka pRC0610)

Plasmid

#200834

Purpose

Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSE901

Plasmid

#91709

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-LacI

Plasmid

#183921

Purpose

Lac repressor labeled with tdTomato; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdTomato-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-O-GECO1

Plasmid

#46025

Purpose

Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

O-GECO1

Expression

Mammalian

Mutation

R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and TurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Nme2sgRNA_pLKO

Plasmid

#119926

Purpose

Nme2Cas9 U6-driven sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

NmeCas9 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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