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Showing: 201 - 220 of 583 results

  1. pCAG-GFP-PQRv3-RFP

    Plasmid
    #73951
    Purpose
    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.
    Depositor
    Brian Chen
    Article
    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do
    Inserts
    sfGFP (superfolder GFP)
    RFP
    Use
    Tags
    A residual PQR peptide wii be left at the C-termi…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin and Chicken beta actin (shared…
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only

  4. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid
    #84561
    Purpose
    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
    Depositor
    Robert Singer
    Article
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    Use
    Lentiviral
    Tags
    Flag, Suntag, oxBFP, AID (auxin-inducible degron)
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    84561-plasmid-map-sequence-id-331246

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  5. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Article
    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131132-plasmid-map-sequence-id-255500

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  8. pCDH-CB-iCre

    Plasmid
    #72257
    Purpose
    Express iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    iCre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available since
    Jan. 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72257-plasmid-map-sequence-id-308172

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  9. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid
    #58993
    Purpose
    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
    Depositor
    Snorri Thorgeirsson
    Article
    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31
    Inserts
    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EcoRV site in MCS converted to BamHI site
    Promoter
    CMV enhancer-chicken beta actin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    58993-plasmid-map-sequence-id-457463

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  11. pODN-mR2-ACTB

    Plasmid
    #183868
    Purpose
    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    ACTB homology arms with mRuby2-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183868-plasmid-map-sequence-id-362451

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  15. pBS_GGSG_mAID_HA_BactBsd

    Plasmid
    #235504
    Purpose
    single mAID_3xHA cloning vector with Roxed Bact-Bsd
    Depositor
    Brian Hendrich
    Insert
    mini-Auxin Inducible Degron
    Use
    Mouse Targeting
    Tags
    mAID, HA
    Expression
    Mutation
    None
    Promoter
    beta actin
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235504-plasmid-map-sequence-id-474046

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  16. pCAX-SCLM

    Plasmid
    #216757
    Purpose
    For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CAG (cytomegalovirus-chicken beta-actin-rabbit be…
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216757-plasmid-map-sequence-id-429395

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  17. pSox2_Halo_Ty2_BactBsd

    Plasmid
    #235513
    Purpose
    C-term HALO targeting construct
    Depositor
    Brian Hendrich
    Insert
    Sox2 (Sox2 Mouse)
    Use
    Mouse Targeting
    Tags
    HALO, 2xTy
    Expression
    Mutation
    None
    Promoter
    beta actin
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235513-plasmid-map-sequence-id-473982

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  18. ATSR

    Plasmid
    #50052
    Purpose
    expresses t.gondii VWA+TSR1 domains in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2-ATSR
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    S158A
    Promoter
    Chick beta-actin promoter
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pBIR-GFP

    Plasmid
    #49807
    Purpose
    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.
    Depositor
    Thanos Halazonetis
    Insert
    GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    beta-actin
    Available since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    49807-plasmid-map-sequence-id-74303

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  20. pCDH-CB-copGFP-T2A-iCre

    Plasmid
    #72256
    Purpose
    Express copGFP and iCre under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-iCre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72256-plasmid-map-sequence-id-134470

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Showing: 201 - 220 of 583 results