Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,169 results

Showing: 5551 - 5600 of 164169 results

  1. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

    Enlarge

  3. pKL2299A

    Plasmid
    #222105
    Purpose
    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.
    Depositor
    Kan Wang
    Article
    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.
    Insert
    virA
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    virA promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222105-plasmid-map-sequence-id-442317

    Enlarge

  5. TFORF1652

    Plasmid
    #143532
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SPI1 (SPI1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143532-plasmid-map-sequence-id-400377

    Enlarge

  8. MC177VSG221RNAi

    Plasmid
    #59730
    Purpose
    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
    Depositor
    Gloria Rudenko
    Article
    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10
    Insert
    VSG221 RNAi fragment
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    opposing T7 promoters
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59730-plasmid-map-sequence-id-93746

    Enlarge

  11. pITGA4-GFP

    Plasmid
    #81178
    Purpose
    Mammalian expression of human full length ITGA4-EGFP
    Depositor
    Chinten James Lim
    Insert
    ITGA4 (ITGA4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Contains KLGFFKR motif; A1002L (please see deposi…
    Promoter
    CMV
    Available since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    81178-plasmid-map-sequence-id-175302

    Enlarge

  15. pHH0103 IQGAP1 WW domain

    Plasmid
    #104279
    Purpose
    Bacterial expression of WW domain from IQGAP1
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    IQGAP1 WW domain (IQGAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104279-plasmid-map-sequence-id-203685

    Enlarge

  17. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179430
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-HS4-mch (SH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179430-plasmid-map-sequence-id-355528

    Enlarge

  22. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

    Viral Prep
    #73633-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>
    Depositor
    John Doench, David Root
    Article
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Promoter
    Tags
    None
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  23. pEZYHb

    Plasmid
    #18669
    Purpose
    pKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Tags
    hemoglobin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18669-plasmid-map-sequence-id-8813

    Enlarge

  24. Pet28a_Human_H3.1

    Plasmid
    #42631
    Depositor
    Joe Landry
    Insert
    Human H3.1 (H3C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42631-plasmid-map-sequence-id-57746

    Enlarge

  30. pDEW8

    Plasmid
    #84238
    Purpose
    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae
    Depositor
    David Weinberg
    Article
    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1
    Insert
    HA-mRuby-P2A-eGFP
    Use
    Tags
    HA-mRuby and eGFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84238-plasmid-map-sequence-id-164972

    Enlarge

  37. XP12 Xbra

    Plasmid
    #16978
    Depositor
    Randall Moon
    Insert
    Xbra (t.S Frog)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  38. 458 p5E-uasE1b

    Plasmid
    #26026
    Depositor
    Nathan Lawson
    Insert
    E1B basal promoter + UAS repeats
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    26026-plasmid-map-sequence-id-12211

    Enlarge

Showing: 5551 - 5600 of 164169 results