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169,450 results

Showing: 5551 - 5600 of 169450 results

  2. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  3. LgBiT-LentiB3 Transfer Vector

    Plasmid
    #236912
    Purpose
    Lentiviral packaging plasmid for generation of LgBiT lentiviral particles to transduce HiBiT cell lines
    Depositor
    Promega Corporation
    Has Service
    DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    LgBiT
    Mutation
    None
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236912-plasmid-map-sequence-id-474760

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  5. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  7. pLCKO2::TKOv3

    Pooled Library
    #125517
    Purpose
    Human CRISPR lentiviral library containing 70,948 guides (4-5 guides/gene) targeting 18,053 human protein-coding genes, in a non-Cas9 containing backbone
    Depositor
    Jason Moffat
    Article
    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  9. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  14. pYPQ-dzCas9-Act3.0

    Plasmid
    #158414
    Purpose
    CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dzCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    158414-plasmid-map-sequence-id-332091

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  20. FRT_htr2c(c)-GFP

    Plasmid
    #79677
    Purpose
    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP
    Depositor
    Stefan Stamm
    Article
    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do
    Insert
    serotonin receptor 2C (HTR2C Human)
    Tags
    EGFP in Frame
    Expression
    Mammalian
    Mutation
    splice site of exon Vb in consensus
    Promoter
    CMV
    Available Since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pHIPH4

    Plasmid
    #117685
    Purpose
    Hansenula polymorpha expression plasmid
    Depositor
    Ida van der Klei
    Insert
    Alcohol Oxidase promoter
    Expression
    Yeast
    Promoter
    Alcohol Oxidase
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    117685-plasmid-map-sequence-id-232223

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  29. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  30. TFORF2857

    Plasmid
    #143642
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ID4 (ID4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143642-plasmid-map-sequence-id-396968

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  37. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  40. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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  41. DDR_MKOv4

    Pooled Library
    #140219
    Purpose
    Knockout library targeting human DNA damage response and DNA repair genes.
    Depositor
    Junjie Chen
    Article
    Su et al DNA Repair (Amst). 2020 Jan 16;87:102803
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  46. pCI-Puro_hCOL4A4-Myc-His

    Plasmid
    #198093
    Purpose
    Express hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198093-plasmid-map-sequence-id-389398

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  50. pGWB508-cTP-mCherry

    Plasmid
    #213862
    Purpose
    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.
    Insert
    Plastid transit peptide fused mCherry-UnaG
    Expression
    Bacterial and Plant
    Available Since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213862-plasmid-map-sequence-id-425330

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Showing: 5551 - 5600 of 169450 results