We narrowed to 17,355 results for: She

1. PB CAG-sfGFP-T2A-iCreERT2

   Plasmid

   #177309

   Purpose

   CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-iCreERT2
   

   Use

   Cre/Lox; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA_Lifeact-GFP_NLS-mCherry

   Plasmid

   #69058

   Purpose

   Dual Fluorescent Reporter for F-actin and Nuclei

   Depositor

   Olivier Pertz

   Insert

   LifeAct-GFP_IRES_NLS-mCherry
   

   Use

   Tags

   Lifeact-GFP, NLS-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTBY1-T4uvsY

   Plasmid

   #228214

   Purpose

   Expression in BL21(DE3) of T4 UvsY-like recombination mediator

   Depositor

   Stephen Benkovic

   Insert

   uvsY (uvsY Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExp-mCherry

   Plasmid

   #112569

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis, Strep II, and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJA54 HLA-A*03:01

   Plasmid

   #180451

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-A*03:01
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

   Plasmid

   #198071

   Purpose

   The AAV vector for iRFP713(V256C) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP713(V256C)
   

   Use

   AAV

   Tags

   P2A-EGFP

   Expression

   Mutation

   V256C mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. nuc-mCherry-pTRIP

   Plasmid

   #163520

   Purpose

   Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   nucleus targeted mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGHE-PdNIP2-1

   Plasmid

   #108447

   Purpose

   PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

   Depositor

   Richard Bélanger

   Insert

   PdNIP2-1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-ymScarletI-SpHis5

   Plasmid

   #219844

   Purpose

   tagging vector to tag genes with ymScarletI and a HIS5 gene

   Depositor

   Bas Teusink

   Insert

   ymScarletI-SpHis5
   

   Use

   Tags

   ymScarletI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCEV-G2-Km ymNeongreen

    Plasmid

    #193959

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExp-Bla

    Plasmid

    #112561

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-Bla and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AbVec_IGHC

    Plasmid

    #183701

    Purpose

    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGHG1 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pExp-IF2

    Plasmid

    #112567

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-IF2 and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-syn-DIO-axon-jGCaMP8f

    Plasmid

    #220531

    Purpose

    For Cre-dependent enriched expression of GCaMP8f in axons

    Depositor

    Lin Tian

    Insert

    Axon-DIO-GCaMP8f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-N2cL

    Plasmid

    #100812

    Purpose

    express rabies virus CVS N2c strain polymerase gene (N2cL)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain polymerase gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-N2cN

    Plasmid

    #100801

    Purpose

    express rabies virus CVS N2c strain nucleoprotein gene (N2cN)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain nucleoprotein gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-N2cP

    Plasmid

    #100808

    Purpose

    express rabies virus CVS N2c strain phosphoprotein gene (N2cP)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain phosphoprotein gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AbVec_IGKC

    Plasmid

    #183702

    Purpose

    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGKC (IGKC )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AbVec_IGLC

    Plasmid

    #183703

    Purpose

    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGLC2 (IGLC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AbVec2.0-mIghg3

    Plasmid

    #127160

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg3 (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLIX403-EYFP-ccdB-Hygro

    Plasmid

    #158555

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV.TBG.PI.FLPo.rBG

    Plasmid

    #236288

    Purpose

    AAV vector expressing FLPo from TBG promoter

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TBG

    Available since

    May 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pTCF236 HLA-B*40:01

    Plasmid

    #180463

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*40:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET32a-Trx-His6-TEV_SacB

    Plasmid

    #164588

    Purpose

    Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

    Depositor

    Ernst Schonbrunn

    Insert

    TEV-SacB
    

    Use

    Tags

    Trx-His6 followed by TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBABE.is4-mOpn

    Plasmid

    #74410

    Purpose

    Expresses mouse Osteopontin from transcript tv4 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Insert

    mouse Opn (Spp1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pD451-pA-APEX2

    Plasmid

    #157863

    Purpose

    Bacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labeling

    Depositor

    Alex Kentsis

    Insert

    pA-APEX2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTCF7 HLA-B*07:02

    Plasmid

    #180446

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*07:02
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Use

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCIG3 Rab5CA

    Plasmid

    #78268

    Purpose

    Rab5 Constitutively active (Q79L) expression

    Depositor

    Felicia Goodrum

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pExp-sfGFP

    Plasmid

    #112573

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNBU2_repA1

    Plasmid

    #211910

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. tet-pLKO.puro_shLuc

    Plasmid

    #110422

    Purpose

    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    Luciferase
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO (RNA PolIII)

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid

    #191442

    Purpose

    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hROSA26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pB6-Hipp11-DTA-Capecchi-for

    Plasmid

    #126649

    Purpose

    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid

    #72259

    Purpose

    Express FLPe and tdTomato under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T230I in tdTomato (please see depositor's co…

    Promoter

    CB promoter and chicken beta-actin + CMV enhancer

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge