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Plasmid#134777Purposemammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signalingDepositorInsertSponGee-Kras
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
M77 pIRESpuro-GLUE-hDsh3
Plasmid#15102DepositorInsertDsh3 (DVL3 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159845PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-gp78/JM20
Plasmid#13303DepositorInsertgp78 (AMFR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shFXN_2
Plasmid#102971PurposeSuppression of FXNDepositorInsertshFXN_2 (FXN Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
HMGA2-FLAG
Plasmid#25409DepositorInserthigh mobility group AT-Hook 2 (Hmga2 Mouse)
UseRetroviral; ChipTagsFLAGExpressionMammalianMutationNonePromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F3
Plasmid#55786PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)DepositorInsert3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM4951 (pCV0063)
Plasmid#120084PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
UseTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorInsertsSpCas9
PmCDA1
UseTagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-GST-LC3B
Plasmid#216836PurposeFor expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)DepositorInsertLC3B (Gly exposed) (MAP1LC3B Human)
UseTagsGSTExpressionBacterialMutationPromoterTAC promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dnmt3b-R
Plasmid#122333PurposeExpresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dnmt3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mEGFP-NLS
Plasmid#205005PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP-NLS
Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mAvicFP1
Plasmid#129512PurposeExpresses LifeAct-mAvicFP1 in mammalian cellsDepositorInsertLifeAct-mAvicFP1
UseTagsExpressionMammalianMutationmAvicFP1 is AvicFP1 F64L + A206KPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-Gsk3b(new)
Plasmid#122341PurposeExpresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cellsDepositorInsertsgRNA for mouse Gsk3b
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NO- DSD Sensor 3
Plasmid#199787PurposeSynthetic gene circuitDepositorInsertNO- DSD Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F6
Plasmid#55789PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)DepositorInsert3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorInsertmCherry-LBK1(K78I) (STK11 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159858PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_APOBEC1-UdgX-nCas9
Plasmid#163531PurposeMammalian CG-to-GC base editingDepositorInsertAPOBEC1-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 A domain (human)
Plasmid#47413DepositorInsertIntersectin SH3 domain A (ITSN1 Human)
UseTagsGSTExpressionBacterialMutationonly SH3 domain APromotertacAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-mouse Plaat3 (N157stop)
Plasmid#168771PurposeExpresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cellsDepositorInsertHrasls3 (Plaat3 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3 4KR
Plasmid#78813PurposeTo overexpress RIPK3 4KR in Mammalian CellDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationK55R, K89R, K363R, K501RPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE
Plasmid#120268PurposePermits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporterDepositorInsertEF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F5
Plasmid#55788PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)DepositorInsert3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_EE-nCas9
Plasmid#163541PurposeMammalian CG-to-GC base editingDepositorInsertEE-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_LHX2
Plasmid#167776PurposeExpresses LHX2 in mammalian cellsDepositorInsertLHX2 (LHX2 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST_CAHS3_HYPDU (pBS0333)
Plasmid#185179PurposeFor the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertCAHS3_HYPDU
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_APOE_HUMAN_E114I (pBS0839)
Plasmid#185274PurposeFor the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAPOE_HUMAN_E114I
UseTagsExpressionMammalianMutationE114IPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 p18 mito
Plasmid#26638DepositorInsertp18 mito (LAMTOR1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-bJun-C-mSOG-IRES-mCherry
Plasmid#136600PurposeEncodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).DepositorInsertbJun-C-mSOG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159862PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Trib3
Plasmid#131157PurposeMammalian expression of flag-tagged mouse TRIB3DepositorInsertTrib3 (Trib3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-POLI-ST2-com vector
Plasmid#176234PurposeVector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replaceDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(1363-1370Mut)-MBP
Plasmid#190038PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and MBPExpressionMammalianMutation1363 RDKDLIES 1370 to GSSGGSSGPromoterCMVAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only